Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits

NWIPB OpenIR

	Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits
	Li, Guo-Liang ; You, Jin-Mao ; Song, Cui-Hua ; Xia, Lian ; Zheng, Jie ; Suo, You-Rui
	2011-04-13
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ; Li, GL; You, JM; Song, CH; Xia, LA; Zheng, J; Suo, YR.Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits,JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2011,59(7):2972-2979
摘要	Triterpenic acids are widespread in plants and have multiplicity of biological properties. Unfortunately the method for accurate analysis of these compounds remains poorly investigated. This study proposed a highly sensitive and selective precolumn derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescent labeling reagent by HPLC with fluorescence detection (FLD). Response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labeling of the analyzed components. The rapid separation of five triterpenic acids could be achieved in as little as 16 mm. This developed method offered the exciting detection limits of 1.68-2.04 ng/mL. When applied to several popular fruits in China, it revealed satisfactory applicability and reproducibility. This developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products.; Triterpenic acids are widespread in plants and have multiplicity of biological properties. Unfortunately the method for accurate analysis of these compounds remains poorly investigated. This study proposed a highly sensitive and selective precolumn derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescent labeling reagent by HPLC with fluorescence detection (FLD). Response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labeling of the analyzed components. The rapid separation of five triterpenic acids could be achieved in as little as 16 mm. This developed method offered the exciting detection limits of 1.68-2.04 ng/mL. When applied to several popular fruits in China, it revealed satisfactory applicability and reproducibility. This developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/15359
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, Guo-Liang,You, Jin-Mao,Song, Cui-Hua,et al. Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, Li, GL; You, JM; Song, CH; Xia, LA; Zheng, J; Suo, YR.Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits,JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2011,59(7):2972-2979,2011.
APA	Li, Guo-Liang,You, Jin-Mao,Song, Cui-Hua,Xia, Lian,Zheng, Jie,&Suo, You-Rui.(2011).Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Li, Guo-Liang,et al."Development of a New HPLC Method with Precolumn Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2011).