Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography

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	Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography
	Jia Jing ; Li Yu-Lin ; Zhao Xiao-Hui ; Xiao Yuan-Can ; Chen Gui-Chen ; You Jin-Mao ; Wei Li-Xin
	2011-04-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ; Jia, J; Li, YL; Zhao, XH; Xiao, YC; Chen, GC; You, JM; Wei, LX.Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(4):584-587
摘要	High speed counter-current chromatographic (HSCCC) method was used to purify and separate methylswertianin, gentiacaulein and decussatin from Swertia mussotti Franch. with a two-phase solvent system composed of hexane-ethyl acetate-ethanol-water (5 : 5 : 6 : 4, V/V). The upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow rate of 2.5 mL/min. The experimental conditions were controlled at 850 r/min and 25 degrees C and the effluent was detected at 254 nm. The separation producs of methylswertianin, gentiacaulein and decussatin with the purity of over 98% were obtained by high performance liquid chromatography, and identification of their structures was performed by (1)H NMR, (13)C NMR and 2D NMR.; High speed counter-current chromatographic (HSCCC) method was used to purify and separate methylswertianin, gentiacaulein and decussatin from Swertia mussotti Franch. with a two-phase solvent system composed of hexane-ethyl acetate-ethanol-water (5 : 5 : 6 : 4, V/V). The upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow rate of 2.5 mL/min. The experimental conditions were controlled at 850 r/min and 25 degrees C and the effluent was detected at 254 nm. The separation producs of methylswertianin, gentiacaulein and decussatin with the purity of over 98% were obtained by high performance liquid chromatography, and identification of their structures was performed by (1)H NMR, (13)C NMR and 2D NMR.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/15364
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jia Jing,Li Yu-Lin,Zhao Xiao-Hui,et al. Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, Jia, J; Li, YL; Zhao, XH; Xiao, YC; Chen, GC; You, JM; Wei, LX.Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(4):584-587,2011.
APA	Jia Jing.,Li Yu-Lin.,Zhao Xiao-Hui.,Xiao Yuan-Can.,Chen Gui-Chen.,...&Wei Li-Xin.(2011).Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Jia Jing,et al."Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2011).