Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification

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	Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification
	Sun, Zhiwei 2,4; You, Jinmao 1,2; Li, Guoliang 2,4; Zhao, Xian'en 3; Suo, Yourui 2; Wang, Xiao 3
	2011-05-15
发表期刊	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN	1570-0232
卷号	879 期号:17-18 页码:1367-1374
文章类型	Article
摘要	A high performance liquid chromatography method for the determination of 20 amino acids (AAs), using 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate (DBCEOC-CI) as a novel fluorescent labeling reagent, has been developed and applied for the analysis of AAs in rat brain microdialysate. The simultaneous separation of 20 AA derivatives was achieved on a Hypersil BDS C(18) column with gradient elution. And the identification of AA derivatives was carried out by on-line electrospray ionization mass spectrometry in positive ion mode. The AA derivatives were detected with excitation and emission at 300 nm and 395 nm, respectively. Excellent linear responses were observed with coefficients of >0.9988. The detection limits ranged from 0.217 to 4.75 nmol/L, at a signal-to-noise ratio of 3. The intra-day and inter-day precision for each AA was <3.2% and <4.3%. respectively. The mean recoveries for all AAs studied were in the range of 92.5-105.6%. Good compositional data could be obtained from as little as 15 mu L of microdialysate. Facile DBCEOC-CI derivatization coupled with high performance liquid chromatography and fluorescence detection allowed the development of a highly sensitive method for the quantitative analysis of trace levels of AAs from microdialysate. (C) 2011 Elsevier B.V. All rights reserved.; A high performance liquid chromatography method for the determination of 20 amino acids (AAs), using 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate (DBCEOC-CI) as a novel fluorescent labeling reagent, has been developed and applied for the analysis of AAs in rat brain microdialysate. The simultaneous separation of 20 AA derivatives was achieved on a Hypersil BDS C(18) column with gradient elution. And the identification of AA derivatives was carried out by on-line electrospray ionization mass spectrometry in positive ion mode. The AA derivatives were detected with excitation and emission at 300 nm and 395 nm, respectively. Excellent linear responses were observed with coefficients of >0.9988. The detection limits ranged from 0.217 to 4.75 nmol/L, at a signal-to-noise ratio of 3. The intra-day and inter-day precision for each AA was <3.2% and <4.3%. respectively. The mean recoveries for all AAs studied were in the range of 92.5-105.6%. Good compositional data could be obtained from as little as 15 mu L of microdialysate. Facile DBCEOC-CI derivatization coupled with high performance liquid chromatography and fluorescence detection allowed the development of a highly sensitive method for the quantitative analysis of trace levels of AAs from microdialysate. (C) 2011 Elsevier B.V. All rights reserved.
关键词	Amino Acid Derivatization High Performance Liquid Chromatography 1 Fluorescence Detection 2 Electrospray Mass Spectrometer 5 6-dibenzocarbazole-9-ethyl Chloroformate
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	PRECOLUMN DERIVATIZATION ; BIOGENIC-AMINES ; CAPILLARY-ELECTROPHORESIS ; INFERIOR COLLICULUS ; CHLORIDE ; QUANTITATION ; SALICYLATE ; POLYAMINES ; PEPTIDES ; MATRIX
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000291131900019
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1546
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Prov Key Lab Life Organ Anal, Qufu, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China 3.Shandong Acad Sci, Shandong Anal & Test Ctr, Jinan, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhiwei,You, Jinmao,Li, Guoliang,et al. Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(17-18):1367-1374.
APA	Sun, Zhiwei,You, Jinmao,Li, Guoliang,Zhao, Xian'en,Suo, Yourui,&Wang, Xiao.(2011).Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(17-18),1367-1374.
MLA	Sun, Zhiwei,et al."Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent by high performance liquid chromatographic fluorescence detection and mass spectrometric identification".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.17-18(2011):1367-1374.