Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning

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	Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning
	Yu, H. H.1,2,5; Guo, Z. L.1,3; Guo, S. C.1; Qi, D. L.4; Yang, X. L.1,5; Yue, P. P.1,5; Cao, H.1,5; Xie, L.1,5; Zhao, X. Q.1
	2011-03-21
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	10 期号:12 页码:2360-2365
文章类型	Article
摘要	Interspecies somatic cell nuclear transfer (iSCNT) offers the possibility of preserving endangered species. Handmade cloning (HMC) has proved to be an efficient alternative to the traditional micromanipulator-based technique in some domestic animal species. This study investigates the possibility of reconstructing the embryos of Tibetan antelope Pantholops hodgsoni by HMC and reports the development of cloned embryos. Fibroblast cells derived from ear tissue from a female Tibetan antelope 3 h after death were used as nuclear donors, and iSCNT was performed using goat oocytes matured in vitro as recipients. A total of 118 embryos were produced by HMC with a cleavage rate of 70.14% and morula formation rate of 100%. However, no blastocysts were obtained in vitro. These results show that somatic cell nuclei of Tibetan antelope can be reprogrammed by goat oocytes. We also demonstrate that HMC is an efficient method of iSCNT using Tibetan antelope nuclear donors. Although the reconstructed embryos did not develop into blastocysts, the morula can be transferred into the womb of a recipient to continue development. Thus, we established an efficient method of iSCNT for producing transplantable embryos in Tibetan antelope by HMC.; Interspecies somatic cell nuclear transfer (iSCNT) offers the possibility of preserving endangered species. Handmade cloning (HMC) has proved to be an efficient alternative to the traditional micromanipulator-based technique in some domestic animal species. This study investigates the possibility of reconstructing the embryos of Tibetan antelope Pantholops hodgsoni by HMC and reports the development of cloned embryos. Fibroblast cells derived from ear tissue from a female Tibetan antelope 3 h after death were used as nuclear donors, and iSCNT was performed using goat oocytes matured in vitro as recipients. A total of 118 embryos were produced by HMC with a cleavage rate of 70.14% and morula formation rate of 100%. However, no blastocysts were obtained in vitro. These results show that somatic cell nuclei of Tibetan antelope can be reprogrammed by goat oocytes. We also demonstrate that HMC is an efficient method of iSCNT using Tibetan antelope nuclear donors. Although the reconstructed embryos did not develop into blastocysts, the morula can be transferred into the womb of a recipient to continue development. Thus, we established an efficient method of iSCNT for producing transplantable embryos in Tibetan antelope by HMC.
关键词	Interspecies Somatic Cell Nuclear Transfer Tibetan Antelope Chiru Handmade Cloning
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	BUFFALO BUBALUS-BUBALIS ; CELL NUCLEAR TRANSFER ; IN-VITRO PRODUCTION ; RABBIT CLONED EMBRYOS ; SOMATIC-CELLS ; BOVINE OOCYTES ; BLASTOCYSTS ; PANDA ; CRYOPRESERVATION ; PREGNANCIES
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000290094900022
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1570
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Yulin Univ, Shannxi Engn Tech Res Ctr Shanbei Cashmere Goat, Yulin 719000, Peoples R China 3.Qinghai Univ Nationality, Acad Chem & Life Sci, Xining 810007, Peoples R China 4.Qinghai Univ, Agr & Anim Husb Coll, Xining 810003, Peoples R China 5.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yu, H. H.,Guo, Z. L.,Guo, S. C.,et al. Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(12):2360-2365.
APA	Yu, H. H..,Guo, Z. L..,Guo, S. C..,Qi, D. L..,Yang, X. L..,...&Zhao, X. Q..(2011).Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(12),2360-2365.
MLA	Yu, H. H.,et al."Interspecies embryo reconstruction in Tibetan antelope Pantholops hodgsonii by handmade cloning".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.12(2011):2360-2365.

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