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Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection
Wang, Honglun1,2; Ou, Lily M. L.1; Suo, Yourui2; Yu, Hua-Zhong1
2011-03-01
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
卷号83期号:5页码:1557-1563
文章类型Article
摘要A method for the convenient detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilization of Pb(2+) -specific DNAzyme sensing constructs on the "transparent side" of a conventional CD-R via mild surface reactions, the Pb(2+) concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a prerecorded audio file). The reading errors increase monotonically over a wide range of Pb(2+) concentrations (from 10 nM to 1 mM), and the selectivity is confirmed by testing several other divalent cations (Zn(2+), Ba(2+), Mg(2+), Ca(2+), Cu(2+), and Hg(2+)),; A method for the convenient detection of lead at the parts-per-billion (ppb)-level has been developed; it uses a conventional compact disc (CD) as the platform for preparing DNAzyme assays and an unmodified optical drive of ordinary desktop/laptop computers as the readout device. In particular, by immobilization of Pb2+ -specific DNAzyme sensing constructs on the "transparent side" of a conventional CD-R via mild surface reactions, the Pb2+ concentration can be determined by a free diagnostic program that checks the error distribution on the CD (i.e., it extracts the number of errors in a prerecorded audio file). The reading errors increase monotonically over a wide range of Pb2+ concentrations (from 10 nM to 1 mM), and the selectivity is confirmed by testing several other divalent cations (Zn2+, Ba2+, Mg2+, Ca2+, Cu2+, and Hg2+),
WOS标题词Science & Technology ; Physical Sciences
关键词[WOS]ATOMIC-ABSORPTION-SPECTROMETRY ; IN-VITRO SELECTION ; RNA-CLEAVING DEOXYRIBOZYME ; CATALYTIC BEACON SENSOR ; GOLD NANOPARTICLES ; WATER SAMPLES ; METAL-IONS ; DNA ; FLUORESCENCE ; SENSITIVITY
收录类别SCI
语种英语
WOS研究方向Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000287685800012
引用统计
被引频次:38[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1582
专题中国科学院西北高原生物研究所
作者单位1.Simon Fraser Univ, Dept Chem, Burnaby, BC V5A 1S6, Canada
2.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Qinghai, Peoples R China
推荐引用方式
GB/T 7714
Wang, Honglun,Ou, Lily M. L.,Suo, Yourui,et al. Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection[J]. ANALYTICAL CHEMISTRY,2011,83(5):1557-1563.
APA Wang, Honglun,Ou, Lily M. L.,Suo, Yourui,&Yu, Hua-Zhong.(2011).Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection.ANALYTICAL CHEMISTRY,83(5),1557-1563.
MLA Wang, Honglun,et al."Computer-Readable DNAzyme Assay on Disc for ppb-Level Lead Detection".ANALYTICAL CHEMISTRY 83.5(2011):1557-1563.
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