瑞香狼毒多糖中单糖组成的毛细管区带电泳分析

NWIPB OpenIR

	瑞香狼毒多糖中单糖组成的毛细管区带电泳分析
	孙志伟 ; 王延宝 ; 白新伟 ; 夏莲 ; 索有瑞
	2010
发表期刊	分析试验室 ; 孙志伟;王延宝;白新伟;夏莲;索有瑞.瑞香狼毒多糖中单糖组成的毛细管区带电泳分析,分析试验室,2010,9(6):6-9
摘要	采用1-苯基-3-甲基-5-吡唑啉酮(PMP) 和1-(2-萘基)-3-甲基-5-吡唑啉酮(NMP) 为衍生试剂, 以反相高效液相色谱(RP2HPLC) 和毛细管区带电泳(CZE)法对9 种单糖衍生物进行了分离研究, 对比确定了NMP 衍生化CZE 法在植物多糖单糖组成分析中的优越性。以NMP 为柱前标记试剂, 建立了毛细管区带电泳定量测定9 种单糖的新方法。方法具有较好的重复性(相对标准偏差RSD 小于4.3 %) , 单糖衍生物检测限为0185～116μmol/L , 回收率为9614 %～104 %。已应用于瑞香狼毒多糖样品的单糖组成分析。 Abstract : Reversed-phase high-performance liquid chromatographic (RP-HPLC) and capillary zone electrophoretic(CZE) methods are compared for the determination of mono-saccharides using 1-phenyl-3-methyl-5-pyrazolone (PMP)and 1-(2-naphthyl)-3-methyl-5-pyrazolone (NMP) as derivatization reagents. In comparison with HPLC , the CEmethod is cheaper and faster (16 min) and the separation is more efficient . The optimum operating conditions are set in 50 mmol/L borate buffer (pH 9. 50) at a voltage of 22 kV and a column temperature of 20 ℃with 10 s injection at atmospheric pressure and detection at 254 nm , respectively. CE method, in conjunction with a zone mode , offers a complete baseline resolution for quantitative analysis of nine labeled mono2saccharide derivatives using NMP as a deri-vatization reagent . Good reproducibilities are obtained with RSD < 4. 3 % , and the limits of detection are in the range of 0. 85～1. 6μmolPL. The proposed method is more efficient and sensitive compared with previously reported method.The mono-saccharides extracted from the root of Stellera chamaejasma L . plant were also determined under the proposed conditions.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/15966
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	孙志伟,王延宝,白新伟,等. 瑞香狼毒多糖中单糖组成的毛细管区带电泳分析[J]. 分析试验室, 孙志伟;王延宝;白新伟;夏莲;索有瑞.瑞香狼毒多糖中单糖组成的毛细管区带电泳分析,分析试验室,2010,9(6):6-9,2010.
APA	孙志伟,王延宝,白新伟,夏莲,&索有瑞.(2010).瑞香狼毒多糖中单糖组成的毛细管区带电泳分析.分析试验室.
MLA	孙志伟,et al."瑞香狼毒多糖中单糖组成的毛细管区带电泳分析".分析试验室 (2010).