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Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu
Hu Xi-gui1,2; Wu Bi-hua1,2; Yan Ze-hong1,2; Liu Deng-cai1,2,3; Wei Yu-ming1,2; Zheng You-liang1,2
2010-11-20
发表期刊AGRICULTURAL SCIENCES IN CHINA
ISSN1671-2927
卷号9期号:11页码:1543-1552
文章类型Article
摘要High molecular weight glutenin subunit (HMW-GS) plays an important role in determining dough property and bread-making quality, and the exploration of novel genes for HMW-GS will be crucial for quality improvement program. A gene coding the y type HMW-GS at Glu-A1 locus in Triticum urartu (AA, 2n = 2x = 14) with an electrophoretic mobility similar to that of 1Dy12, was cloned, sequenced, and heterologously expressed. This novel active 1 Ay gene FJ404595 was confirmed by structure analyses of nucleotide and deduced amino acid sequences combining with phylogenetic analysis. The open reading frame (ORF) of this gene was 1 830 bp, encoded a protein of 608 amino acid residues containing 46 hexapeptides and 14 nonapeptides, which was mostly similar to the 1 Ay gene AM183223 at a high identity of 99.62% with the two substitutions of both leucine/proline and valine/glutamate, obviously different from the I Ay gene EU984504 with 587 residues containing 44 hexapeptides and 13 nonapeptides in T. urartu. The amino acid (leucine) at 446 differed from that (proline) of all the eight compared active 1Ay subunits. The predicted secondary protein structure implied that this 1Ay subunit might also have positive impact on flour processing quality.; High molecular weight glutenin subunit (HMW-GS) plays an important role in determining dough property and bread-making quality, and the exploration of novel genes for HMW-GS will be crucial for quality improvement program. A gene coding the y type HMW-GS at Glu-A1 locus in Triticum urartu (AA, 2n = 2x = 14) with an electrophoretic mobility similar to that of 1Dy12, was cloned, sequenced, and heterologously expressed. This novel active 1 Ay gene FJ404595 was confirmed by structure analyses of nucleotide and deduced amino acid sequences combining with phylogenetic analysis. The open reading frame (ORF) of this gene was 1 830 bp, encoded a protein of 608 amino acid residues containing 46 hexapeptides and 14 nonapeptides, which was mostly similar to the 1 Ay gene AM183223 at a high identity of 99.62% with the two substitutions of both leucine/proline and valine/glutamate, obviously different from the I Ay gene EU984504 with 587 residues containing 44 hexapeptides and 13 nonapeptides in T. urartu. The amino acid (leucine) at 446 differed from that (proline) of all the eight compared active 1Ay subunits. The predicted secondary protein structure implied that this 1Ay subunit might also have positive impact on flour processing quality.
关键词Triticum Urartu 1ay Subunit Expression Electrophoretic Mobility Secondary Structure
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]HIGH-MOLECULAR-WEIGHT ; BREAD-MAKING QUALITY ; GLUTENIN SUBUNIT GENE ; WHEAT STORAGE PROTEINS ; HMW-GLUTENIN ; HETEROLOGOUS EXPRESSION ; AESTIVUM L ; SEQUENCE ; ALLELES ; CLONING
收录类别SCI
语种英语
WOS研究方向Agriculture
WOS类目Agriculture, Multidisciplinary
WOS记录号WOS:000284136800001
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1624
专题中国科学院西北高原生物研究所
作者单位1.Sichuan Agr Univ, Triticeae Res Inst, Wenjiang 611130, Peoples R China
2.Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Yaan 625014, Peoples R China
3.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China
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Hu Xi-gui,Wu Bi-hua,Yan Ze-hong,et al. Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu[J]. AGRICULTURAL SCIENCES IN CHINA,2010,9(11):1543-1552.
APA Hu Xi-gui,Wu Bi-hua,Yan Ze-hong,Liu Deng-cai,Wei Yu-ming,&Zheng You-liang.(2010).Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu.AGRICULTURAL SCIENCES IN CHINA,9(11),1543-1552.
MLA Hu Xi-gui,et al."Characterization of a Novel 1Ay Gene and Its Expression Protein in Triticum urartu".AGRICULTURAL SCIENCES IN CHINA 9.11(2010):1543-1552.
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