Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC

NWIPB OpenIR

	Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC
	Cairang, Limao ; Rui-Suo, Y.
	2012-10-01
发表期刊	ASIAN JOURNAL OF CHEMISTRY ; Luo, JT; Hao, M; Zhang, L; Chen, JX; Zhang, LQ; Yuan, ZW; Yan, ZH; Zheng, YL; Zhang, HG; Yen, Y; Liu, DC.Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC,ASIAN JOURNAL OF CHEMISTRY,2012,24(10):4380-4384
摘要	A novel, sensitive and selective HPLC-FLC method coupled with pre-column derivatization was developed for amino acid determination in Hippophae rhamnoides L. fruit from Qinghai-Tibetan plateau. The method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (DBCEC) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). Detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. Linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. Results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. Proline was dominating among free amino acids in samples, which plays a vital role for Hippophae rhamnoides L. in resisting to drought, tolerating soil salinity and lowing temperature in Qinghai-Tibetan plateau.; A novel, sensitive and selective HPLC-FLC method coupled with pre-column derivatization was developed for amino acid determination in Hippophae rhamnoides L. fruit from Qinghai-Tibetan plateau. The method is based on the derivatization reaction of amino group with 2[2-(dibenzocarbazole)-ethoxylethyl chloroformate (DBCEC) and the derivatives could be measured at 390 nm (excitation wavelength: 300 nm). Detection limits (calculated as the signal-to-noise ratio 3:1) were 12.85-51.68 mol for the labeled amino acids. Linearity was observed with coefficients > 0.9991 and the recoveries ranged from 92.7-105.4 %. Results in this research showed that the amount of essential amino acids accounted for 70.42 % of free amino acids and 22.68 % of the total amino acids, respectively, which benefits people's health through drinking the juice or eating the related products. Proline was dominating among free amino acids in samples, which plays a vital role for Hippophae rhamnoides L. in resisting to drought, tolerating soil salinity and lowing temperature in Qinghai-Tibetan plateau.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/16462
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Cairang, Limao,Rui-Suo, Y.. Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC[J]. ASIAN JOURNAL OF CHEMISTRY, Luo, JT; Hao, M; Zhang, L; Chen, JX; Zhang, LQ; Yuan, ZW; Yan, ZH; Zheng, YL; Zhang, HG; Yen, Y; Liu, DC.Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC,ASIAN JOURNAL OF CHEMISTRY,2012,24(10):4380-4384,2012.
APA	Cairang, Limao,&Rui-Suo, Y..(2012).Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC.ASIAN JOURNAL OF CHEMISTRY.
MLA	Cairang, Limao,et al."Simultaneous Determination of Amino Acids in Hippophae rhamnoides L. with Pre-column Derivatization by HPLC".ASIAN JOURNAL OF CHEMISTRY (2012).