PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC

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	PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC
	Chen, Tao ; Wang, Ping ; Du, Yuzhi ; Shen, Yuhu ; Li, Yulin
	2012
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES ; Zhang, BY; Wang, H; Luo, XD; Du, ZZ; Wu, HF; Shen, JW; Zhang, XF.PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(18):2524-2532
摘要	In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.; In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/16525
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Tao,Wang, Ping,Du, Yuzhi,et al. PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, Zhang, BY; Wang, H; Luo, XD; Du, ZZ; Wu, HF; Shen, JW; Zhang, XF.PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC,JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2012,35(18):2524-2532,2012.
APA	Chen, Tao,Wang, Ping,Du, Yuzhi,Shen, Yuhu,&Li, Yulin.(2012).PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES.
MLA	Chen, Tao,et al."PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES (2012).