A potato NOA gene increased salinity tolerance in Arabidopsis thaliana

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	A potato NOA gene increased salinity tolerance in Arabidopsis thaliana
	Zhang, B.1,2; Wang, H. Q.1; Liu, B. L.1; Liu, J.1,2; Wang, X.1,2; Liu, Q.1,2; Zhang, H. G.1
	2010-09-06
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	9 期号:36 页码:5869-5878
文章类型	Article
摘要	The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.; The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.
关键词	Stnoa1 Transformation Solanum Tuberosum Atnoa1 Mutant Salt Tolerance
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	NITRIC-OXIDE SYNTHASE ; NITRATE REDUCTASE ; STOMATAL CLOSURE ; OXIDATIVE STRESS ; OLD ENZYME ; 2 ECOTYPES ; PLANTS ; IDENTIFICATION ; RESPONSES ; PROTEINS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000281680400014
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1654
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, B.,Wang, H. Q.,Liu, B. L.,et al. A potato NOA gene increased salinity tolerance in Arabidopsis thaliana[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(36):5869-5878.
APA	Zhang, B..,Wang, H. Q..,Liu, B. L..,Liu, J..,Wang, X..,...&Zhang, H. G..(2010).A potato NOA gene increased salinity tolerance in Arabidopsis thaliana.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(36),5869-5878.
MLA	Zhang, B.,et al."A potato NOA gene increased salinity tolerance in Arabidopsis thaliana".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.36(2010):5869-5878.

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