Knowledge Management System of Northwest Institute of Plateau Biology, CAS
A potato NOA gene increased salinity tolerance in Arabidopsis thaliana | |
Zhang, B.1,2; Wang, H. Q.1; Liu, B. L.1; Liu, J.1,2; Wang, X.1,2; Liu, Q.1,2; Zhang, H. G.1 | |
2010-09-06 | |
发表期刊 | AFRICAN JOURNAL OF BIOTECHNOLOGY |
ISSN | 1684-5315 |
卷号 | 9期号:36页码:5869-5878 |
文章类型 | Article |
摘要 | The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.; The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance. |
关键词 | Stnoa1 Transformation Solanum Tuberosum Atnoa1 Mutant Salt Tolerance |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
关键词[WOS] | NITRIC-OXIDE SYNTHASE ; NITRATE REDUCTASE ; STOMATAL CLOSURE ; OXIDATIVE STRESS ; OLD ENZYME ; 2 ECOTYPES ; PLANTS ; IDENTIFICATION ; RESPONSES ; PROTEINS |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biotechnology & Applied Microbiology |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000281680400014 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://210.75.249.4/handle/363003/1654 |
专题 | 中国科学院西北高原生物研究所 |
作者单位 | 1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, B.,Wang, H. Q.,Liu, B. L.,et al. A potato NOA gene increased salinity tolerance in Arabidopsis thaliana[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(36):5869-5878. |
APA | Zhang, B..,Wang, H. Q..,Liu, B. L..,Liu, J..,Wang, X..,...&Zhang, H. G..(2010).A potato NOA gene increased salinity tolerance in Arabidopsis thaliana.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(36),5869-5878. |
MLA | Zhang, B.,et al."A potato NOA gene increased salinity tolerance in Arabidopsis thaliana".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.36(2010):5869-5878. |
条目包含的文件 | 下载所有文件 | |||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
A potato NOA gene in(900KB) | 开放获取 | -- | 浏览 下载 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论