Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification

NWIPB OpenIR

	Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification
	Li, GL; Sun, ZW; Xia, LA; Shi, JY; Liu, YJ; Suo, YR; You, JM
	2010
发表期刊	JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
ISSN	0022-5142
卷号	90 期号:11 页码:1905-1913
摘要	BACKGROUND: Supercritical CO(2) was utilised to extract Chinese star anise seed oil (CSASO), and a three-level Box - Behnken factorial design from response surface methodology was applied to optimise the extraction conditions, including pressure, temperature and amount of modifier (ethanol). The compositional analysis of fatty acids in CSASO was performed by HPLC with fluorescence detection using 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methylbenzenesulfonate (BCETS) as labelling reagent. Identification was carried out by online atmospheric chemical ionisation-mass spectrometry. RESULTS: The optimum extraction conditions were as follows: extraction pressure, 27.72 MPa, extraction temperature, 46.22 degrees C, and amount of modifier, 8.58 vol.%. The experimental result showed that the maximum extraction yield was 25.31 +/- 0.22% (w/w) under the conditions proposed. The compositional analysis indicated that CSASO mainly contained C18: 2, C18 : 1, C18 : 3, C20 :4, C16, C18 and C20 fatty acids. CONCLUSION: In this study, a fast, simple and high-efficiency supercritical technique for extracting oil from Chinese star anise seed was developed. Simultaneous determination of fatty acids in CSASO using BCETS as the labelling reagent with HPLC fluorescence detection and online mass spectroscopy identification has been successfully achieved. (C) 2010 Society of Chemical Industry
关键词	Chinese Star Anise Seed Oil Supercritical Co(2) Fluid Extraction Response Surface Methodology Fatty Acids Hplc/apci/ms
收录类别	sci
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1656
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Li, GL,Sun, ZW,Xia, LA,et al. Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,2010,90(11):1905-1913.
APA	Li, GL.,Sun, ZW.,Xia, LA.,Shi, JY.,Liu, YJ.,...&You, JM.(2010).Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification.JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,90(11),1905-1913.
MLA	Li, GL,et al."Supercritical CO(2) oil extraction from Chinese star anise seed and simultaneous compositional analysis using HPLC by fluorescence detection and online atmospheric CI-MS identification".JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE 90.11(2010):1905-1913.