Involvement of nitric oxide synthase-dependent nitric oxide and exogenous nitric oxide in alleviating NaCl induced osmotic and oxidative stress in Arabidopsis thaliana

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	Involvement of nitric oxide synthase-dependent nitric oxide and exogenous nitric oxide in alleviating NaCl induced osmotic and oxidative stress in Arabidopsis thaliana
	Zhang, Bo 1,2; Wang, Haiqing 1; Wang, Pei 1,2; Zhang, Huaigang 1
	2010-07-04
发表期刊	AFRICAN JOURNAL OF AGRICULTURAL RESEARCH
ISSN	1991-637X
卷号	5 期号:13 页码:1713-1721
文章类型	Article
摘要	To elucidate the roles of endogenous nitric oxide on Arabidopsis thaliana tolerance to salt stress, a moderate concentration of NaCl was applied to wild-type (WT) and mutant (Atnoa1) plants which have an impaired in vivo nitric oxide synthase and reduced endogenous nitric oxide content due to T-DNA insertion in the first exon of the NOA1 gene. The exhibited greater inhibition of root growth, higher leaf water loss (LWL), lower contents of chlorophyll, soluble protein, proline, higher activities of peroxidase (POD), ascorbate peroxide (APX), and gluthinone reductase (GR), it also showed lower activities of superoxide dismutase (SOD) and catalase (CAT) than wild-type plants under NaCl stress. The nitric oxide synthase (NOS) inhibitor NG-nitro-L-Arg (L-NNA) enhanced NaCl induced growth inhibition, osmotic stress, and oxidative stress in wild-type plants. Meanwhile the NO donor, sodium nitroprusside (SNP), alleviated the NaCl induced damages in Atnoa1 plants. These results indicate that both NOS-dependent endogenous NO and exogenous NO were involved in salt resistance in A. thaliana.; To elucidate the roles of endogenous nitric oxide on Arabidopsis thaliana tolerance to salt stress, a moderate concentration of NaCl was applied to wild-type (WT) and mutant (Atnoa1) plants which have an impaired in vivo nitric oxide synthase and reduced endogenous nitric oxide content due to T-DNA insertion in the first exon of the NOA1 gene. The exhibited greater inhibition of root growth, higher leaf water loss (LWL), lower contents of chlorophyll, soluble protein, proline, higher activities of peroxidase (POD), ascorbate peroxide (APX), and gluthinone reductase (GR), it also showed lower activities of superoxide dismutase (SOD) and catalase (CAT) than wild-type plants under NaCl stress. The nitric oxide synthase (NOS) inhibitor NG-nitro-L-Arg (L-NNA) enhanced NaCl induced growth inhibition, osmotic stress, and oxidative stress in wild-type plants. Meanwhile the NO donor, sodium nitroprusside (SNP), alleviated the NaCl induced damages in Atnoa1 plants. These results indicate that both NOS-dependent endogenous NO and exogenous NO were involved in salt resistance in A. thaliana.
关键词	Antioxidant Enzymes Arabidopsis Thaliana Reactive Oxygen Species
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	PLANT SALT TOLERANCE ; SALINITY RESISTANCE ; ACTIVE OXYGEN ; WATER-STRESS ; PROLINE ; GLUTATHIONE ; ASCORBATE ; GROWTH ; CHLOROPLASTS ; RESPONSES
收录类别	SCI
语种	英语
WOS研究方向	Agriculture
WOS类目	Agriculture, Multidisciplinary
WOS记录号	WOS:000280348800022
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1670
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Bo,Wang, Haiqing,Wang, Pei,et al. Involvement of nitric oxide synthase-dependent nitric oxide and exogenous nitric oxide in alleviating NaCl induced osmotic and oxidative stress in Arabidopsis thaliana[J]. AFRICAN JOURNAL OF AGRICULTURAL RESEARCH,2010,5(13):1713-1721.
APA	Zhang, Bo,Wang, Haiqing,Wang, Pei,&Zhang, Huaigang.(2010).Involvement of nitric oxide synthase-dependent nitric oxide and exogenous nitric oxide in alleviating NaCl induced osmotic and oxidative stress in Arabidopsis thaliana.AFRICAN JOURNAL OF AGRICULTURAL RESEARCH,5(13),1713-1721.
MLA	Zhang, Bo,et al."Involvement of nitric oxide synthase-dependent nitric oxide and exogenous nitric oxide in alleviating NaCl induced osmotic and oxidative stress in Arabidopsis thaliana".AFRICAN JOURNAL OF AGRICULTURAL RESEARCH 5.13(2010):1713-1721.