Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS

NWIPB OpenIR

	Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS
	Caidan, Rezeng ; Cairang, Limao ; Liubin ; Suo Yourui ; Liubin (reprint author), Beijing Univ Chinese Med, Dept Chinese Med Chem, Beijing, Peoples R China.
	2013-04-01
发表期刊	ASIAN JOURNAL OF CHEMISTRY ; Caidan, RZ; Cairang, LM; Liubin; Suo, YR.Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS,ASIAN JOURNAL OF CHEMISTRY,2013,25(4):1866-1870
摘要	The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl-4-toluene-sulfonate as labeling reagent. The optimum microwave extraction conditions were as follows: irradiation power 437 W, extraction temperature 75 degrees C and extraction time 12 min. Under these optimized conditions, the maximum oil extraction yield was 5.83 %. All fatty acid derivates had excellent linear responses with correlation coefficients greater than 0.9990. Detection limits (at signal-to-noise of 3:1) were 18.42 to 32.39 pmol. The results indicate that the predominant fatty acids (C10-C22) were 16:0, 18:2, 18:3, 18:0 and 22:0 in total fatty acids. And the unsaturated fatty acids were 18:3, 18:2, 18:1 and 20:4 and reached up to 40.54 % of the total mass contents of fatty acids (C10-C22) in Rubus niveus Thunb. from Qinghai-Tibetan plateau.; The aim of this study was to optimize the microwave extraction conditions of Rubus niveus Thunb. oil from Qinghai-Tibetan plateau via Box-Behnken design of RSM and then analyze the composition of oil by HPLC-MS/MS using 2-(12-oxobenzo[b]acridin-5(12H)-yl)ethyl-4-toluene-sulfonate as labeling reagent. The optimum microwave extraction conditions were as follows: irradiation power 437 W, extraction temperature 75 degrees C and extraction time 12 min. Under these optimized conditions, the maximum oil extraction yield was 5.83 %. All fatty acid derivates had excellent linear responses with correlation coefficients greater than 0.9990. Detection limits (at signal-to-noise of 3:1) were 18.42 to 32.39 pmol. The results indicate that the predominant fatty acids (C10-C22) were 16:0, 18:2, 18:3, 18:0 and 22:0 in total fatty acids. And the unsaturated fatty acids were 18:3, 18:2, 18:1 and 20:4 and reached up to 40.54 % of the total mass contents of fatty acids (C10-C22) in Rubus niveus Thunb. from Qinghai-Tibetan plateau.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/16725
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Caidan, Rezeng,Cairang, Limao,Liubin,et al. Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS[J]. ASIAN JOURNAL OF CHEMISTRY, Caidan, RZ; Cairang, LM; Liubin; Suo, YR.Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS,ASIAN JOURNAL OF CHEMISTRY,2013,25(4):1866-1870,2013.
APA	Caidan, Rezeng,Cairang, Limao,Liubin,Suo Yourui,&Liubin .(2013).Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS.ASIAN JOURNAL OF CHEMISTRY.
MLA	Caidan, Rezeng,et al."Simultaneous Analysis of Fatty Acids in Rubus niveus Thunb. Fruits by HPLC-MS/MS".ASIAN JOURNAL OF CHEMISTRY (2013).