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HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau
Yue, Pengpeng1,2; Sun, Jing1; Zhang, Changxian1,2; Ye, Runrong1; Lu, Xuefeng1; Zhou, Yubi1; Yang, Shibing1,2; Peng, Min1
2010-06-04
Source PublicationJOURNAL OF MEDICINAL PLANTS RESEARCH
ISSN1996-0875
Volume4Issue:11Pages:1053-1058
SubtypeArticle
AbstractThis study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).; This study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).
KeywordHplc-dad Flavonoids Meconopsis Quintuplinervia Tibetan Medicine
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000280356500009
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1680
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Inst Plateau Biol, Res Ctr Natl Tibetan Med, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yue, Pengpeng,Sun, Jing,Zhang, Changxian,et al. HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH,2010,4(11):1053-1058.
APA Yue, Pengpeng.,Sun, Jing.,Zhang, Changxian.,Ye, Runrong.,Lu, Xuefeng.,...&Peng, Min.(2010).HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau.JOURNAL OF MEDICINAL PLANTS RESEARCH,4(11),1053-1058.
MLA Yue, Pengpeng,et al."HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau".JOURNAL OF MEDICINAL PLANTS RESEARCH 4.11(2010):1053-1058.
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