HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau

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	HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau
	Yue, Pengpeng 1,2; Sun, Jing 1; Zhang, Changxian 1,2; Ye, Runrong 1; Lu, Xuefeng 1; Zhou, Yubi 1; Yang, Shibing 1,2; Peng, Min 1
	2010-06-04
发表期刊	JOURNAL OF MEDICINAL PLANTS RESEARCH
ISSN	1996-0875
卷号	4 期号:11 页码:1053-1058
文章类型	Article
摘要	This study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).; This study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).
关键词	Hplc-dad Flavonoids Meconopsis Quintuplinervia Tibetan Medicine
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
收录类别	SCI
语种	英语
WOS研究方向	Pharmacology & Pharmacy
WOS类目	Chemistry, Medicinal
WOS记录号	WOS:000280356500009
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1680
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Res Ctr Natl Tibetan Med, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yue, Pengpeng,Sun, Jing,Zhang, Changxian,et al. HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH,2010,4(11):1053-1058.
APA	Yue, Pengpeng.,Sun, Jing.,Zhang, Changxian.,Ye, Runrong.,Lu, Xuefeng.,...&Peng, Min.(2010).HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau.JOURNAL OF MEDICINAL PLANTS RESEARCH,4(11),1053-1058.
MLA	Yue, Pengpeng,et al."HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau".JOURNAL OF MEDICINAL PLANTS RESEARCH 4.11(2010):1053-1058.