Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes

NWIPB OpenIR

	Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes
	Bai, XW; Song, CH; You, JM; Sun, ZW; Fu, YY; Li, GL
	2010
发表期刊	CHROMATOGRAPHIA
ISSN	0009-5893
卷号	71 期号:11-12 页码:1125-1129
摘要	A simple and mild method for the determination of fatty acids (C(1) - C(10)) based on a condensation reaction using 7-aminonaphthalene-1,3-disulfonic acid (ANDSA) as labeling reagent with capillary zone electrophoresis has been developed. The detection was performed with a diode array detector at 254 nm. A 58.5 cm x 50 mu m i.d. (50 cm effective length) untreated fused-silica capillary was used. To optimize the separation conditions, the background electrolyte concentration, column temperature, voltage and other factors were evaluated. The optimal separation conditions were as follows: 30 mmol L(-1) borate buffer (pH 9.5), 15 mmol L(-1) beta-CD, temperature at 20 A degrees C, pressure 50 mbar and injection time 8 s. Under the established conditions, 10 fatty acid derivatives could be well-separated within 17 min. The linearity was in the range of 0.07-5.0 mu mol L(-1). Detection limits (at a signal-to-noise ratio of 3) were in the range of 0.027-0.042 mu mol L(-1). The fatty acids from the extracted Funaria Hedw. and Selaginella samples were determined with satisfactory results.
关键词	Capillary Zone Electrophoresis Pre-column Derivation Fatty Acids Funaria Hedw. And Selaginella
收录类别	sci
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1682
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Bai, XW,Song, CH,You, JM,et al. Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes[J]. CHROMATOGRAPHIA,2010,71(11-12):1125-1129.
APA	Bai, XW,Song, CH,You, JM,Sun, ZW,Fu, YY,&Li, GL.(2010).Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes.CHROMATOGRAPHIA,71(11-12),1125-1129.
MLA	Bai, XW,et al."Determination of Fatty Acids (C(1)-C(10)) from Bryophytes and Pteridophytes".CHROMATOGRAPHIA 71.11-12(2010):1125-1129.