Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis

NWIPB OpenIR

	Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis
	Hu, Yanping 1,3; Wang, Li 1; Xie, Xiaolong 2; Yang, Jian 1; Li, Yi 1; Zhang, Huaigang 1
	2010-06-01
发表期刊	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
ISSN	0305-1978
卷号	38 期号:3 页码:264-274
文章类型	Article
摘要	Rheum tanguticum is an important but endangered traditional Chinese medicine endemic to China. The wild resources have been declining. Establishing the genetic diversity of the species would assist in its conservation and breeding program. Inter-simple sequence repeats (ISSR) markers were used to assess the genetic diversity and population genetic structure in 13 wild populations of R. tanguticum from Qinghai Province. Thirteen selected primers produced 329 discernible bands, with 326 (92.94%) being polymorphic, indicating high genetic diversity at the species level. The Nei's gene diversity (He) was estimated to be 0.1724 within populations (range 0.1026-0.2104), and 0.2689 at the species level. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly within populations (71.02%), but variance among populations was only 28.98%. In addition, Nei's differentiation coefficients (G(st)) was found to be high (0.3585), confirming the relatively high level of genetic differentiation among populations. Mantel test revealed a significant correlation between genetic and geographic distances (r=0.573, P=0.002), and the unweighted pair-group method using arithmetic average (UPGMA) clustering and Principal coordinates analysis (PCoA) demonstrated similar results. Meanwhile, the genetic diversity of R. tanguticum positively correlated with altitude and annual mean precipitation, but negatively correlated with latitude and annual mean temperature. This result might be an explanation that the natural distribution of R. tanguticum is limited to alpine cold areas. We propose conservation strategy and breeding program for this plant. (C) 2010 Elsevier Ltd. All rights reserved.; Rheum tanguticum is an important but endangered traditional Chinese medicine endemic to China. The wild resources have been declining. Establishing the genetic diversity of the species would assist in its conservation and breeding program. Inter-simple sequence repeats (ISSR) markers were used to assess the genetic diversity and population genetic structure in 13 wild populations of R. tanguticum from Qinghai Province. Thirteen selected primers produced 329 discernible bands, with 326 (92.94%) being polymorphic, indicating high genetic diversity at the species level. The Nei's gene diversity (He) was estimated to be 0.1724 within populations (range 0.1026-0.2104), and 0.2689 at the species level. Analysis of molecular variance (AMOVA) showed that the genetic variation was found mainly within populations (71.02%), but variance among populations was only 28.98%. In addition, Nei's differentiation coefficients (G(st)) was found to be high (0.3585), confirming the relatively high level of genetic differentiation among populations. Mantel test revealed a significant correlation between genetic and geographic distances (r=0.573, P=0.002), and the unweighted pair-group method using arithmetic average (UPGMA) clustering and Principal coordinates analysis (PCoA) demonstrated similar results. Meanwhile, the genetic diversity of R. tanguticum positively correlated with altitude and annual mean precipitation, but negatively correlated with latitude and annual mean temperature. This result might be an explanation that the natural distribution of R. tanguticum is limited to alpine cold areas. We propose conservation strategy and breeding program for this plant. (C) 2010 Elsevier Ltd. All rights reserved.
关键词	Rheum Tanguticum Genetic Diversity Inter-simple Sequence Repeats (Issr) Conservation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	QINGHAI-TIBET PLATEAU ; CHEMICAL-CONSTITUENTS ; DNA MARKERS ; PLANT ; RHUBARB ; RAPD ; CONSERVATION ; POLYMORPHISM ; DIFFERENTIATION ; AMPLIFICATION
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS记录号	WOS:000278709900002
引用统计	被引频次：55[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1688
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Henan Univ Tradit Chinese Med, Zhengzhou 450008, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Hu, Yanping,Wang, Li,Xie, Xiaolong,et al. Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2010,38(3):264-274.
APA	Hu, Yanping,Wang, Li,Xie, Xiaolong,Yang, Jian,Li, Yi,&Zhang, Huaigang.(2010).Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,38(3),264-274.
MLA	Hu, Yanping,et al."Genetic diversity of wild populations of Rheum tanguticum endemic to China as revealed by ISSR analysis".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 38.3(2010):264-274.

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