Genetic diversity and population structure in Meconopsis quintuplinervia (Papaveraceae)

NWIPB OpenIR

	Genetic diversity and population structure in Meconopsis quintuplinervia (Papaveraceae)
	Yang, Shibing 2; Lu, Xuefeng 1; Ye, Runrong 1; Li, Yi 1; Zhou, Yubi 2; Yue, Pengpeng 2; Zhao, Jianzhong 2; Zhang, Changxian 2; Peng, Min 1
	2010-05-24
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	9 期号:21 页码:3048-3053
文章类型	Article
摘要	Meconopsis quintuplinervia is regarded as a valuable medicinal plant in Tibetan medicinal system. This species is distributed in Qinghai, Xizang, Sichuan, Shanxi ,Gansu and Hubei provinces of the People's Republic of China. Genetic variation of 16 M. quintuplinervia populations sampled from Qinghai and Gansu of China was examined by random amplified polymorphic DNA markers (RAPDs). In total, 225 scored DNA bands were amplified from the 17 primers used. Of the 225 loci, 192(85.33%) were polymorphic, and total genetic diversity (Ht) was 0.2954 and Shannon's information index (I) was0.4371, suggesting a relatively high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with PPB, He and I values of 70.50%, 0.2408 and 0.3347, respectively. Analysis of molecular variance (AMOVA) revealed 78.3% of variation within populations and only 21.7% between populations. Nei's coefficient of differentiation (G(ST)) was found to be high (0.2320), also confirming the relatively high level of genetic differentiation within populations. By UPGMA cluster analysis, based on Nei's standard genetic distance, the populations were divided into three groups including the populations distributed in same location together in every group. The results exhibit a strong genetic differentiation which is obviously due to genetic drift in the isolated populations. The genetic structure of M. quintuplinervia has probably been shaped by its breeding modes, biogeographic history and human impact (both grazing and collection for medicinal purposes). This research might be an efficient way to conserve genetic resources of the medicinal plant, in addition to its effective uses.; Meconopsis quintuplinervia is regarded as a valuable medicinal plant in Tibetan medicinal system. This species is distributed in Qinghai, Xizang, Sichuan, Shanxi ,Gansu and Hubei provinces of the People's Republic of China. Genetic variation of 16 M. quintuplinervia populations sampled from Qinghai and Gansu of China was examined by random amplified polymorphic DNA markers (RAPDs). In total, 225 scored DNA bands were amplified from the 17 primers used. Of the 225 loci, 192(85.33%) were polymorphic, and total genetic diversity (Ht) was 0.2954 and Shannon's information index (I) was0.4371, suggesting a relatively high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with PPB, He and I values of 70.50%, 0.2408 and 0.3347, respectively. Analysis of molecular variance (AMOVA) revealed 78.3% of variation within populations and only 21.7% between populations. Nei's coefficient of differentiation (G(ST)) was found to be high (0.2320), also confirming the relatively high level of genetic differentiation within populations. By UPGMA cluster analysis, based on Nei's standard genetic distance, the populations were divided into three groups including the populations distributed in same location together in every group. The results exhibit a strong genetic differentiation which is obviously due to genetic drift in the isolated populations. The genetic structure of M. quintuplinervia has probably been shaped by its breeding modes, biogeographic history and human impact (both grazing and collection for medicinal purposes). This research might be an efficient way to conserve genetic resources of the medicinal plant, in addition to its effective uses.
关键词	Meconopsis Quintuplinervia Regel Genetic Diversity Random Amplified Polymorphic Dna Markers The Qinghai-xizang Plateau Conservation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	AMPLIFIED POLYMORPHIC DNA ; RAPD MARKERS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000278588900005
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1704
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Yang, Shibing,Lu, Xuefeng,Ye, Runrong,et al. Genetic diversity and population structure in Meconopsis quintuplinervia (Papaveraceae)[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(21):3048-3053.
APA	Yang, Shibing.,Lu, Xuefeng.,Ye, Runrong.,Li, Yi.,Zhou, Yubi.,...&Peng, Min.(2010).Genetic diversity and population structure in Meconopsis quintuplinervia (Papaveraceae).AFRICAN JOURNAL OF BIOTECHNOLOGY,9(21),3048-3053.
MLA	Yang, Shibing,et al."Genetic diversity and population structure in Meconopsis quintuplinervia (Papaveraceae)".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.21(2010):3048-3053.