NWIPB OpenIR
A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector
Xia, Lian1,2,3; Sun, Zhi Wei2,3; Li, Guo Liang2,3; Suo, You Rui2; You, Jin Mao1,2
2010-03-01
发表期刊EUROPEAN FOOD RESEARCH AND TECHNOLOGY
ISSN1438-2377
卷号230期号:5页码:715-722
文章类型Article
摘要A rapid and sensitive method was optimized and validated for the separation and quantification of derivatized monosaccharides in polysaccharide from Potentilla anserine L. using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as precolumn derivatization reagent by capillary zone electrophoresis (CZE). On the basis of the optimum conditions, nine NMP-derivatized monosaccharides achieve baseline resolution within 16 min. The developed method has been successfully applied to analyze component monosaccharides of three Potentilla anserine L. samples, which were obtained by gradational precipitation with 50, 70, and 90% aqueous ethanol, respectively. The polysaccharide precipitated from 50% ethanol solvent was composed of fucose, mannose, xylose, glucuronic acid, glucose, rhamnose, galacturonic acid, galactose, arabinose in molar proportion of 1:1.65:1.99:5.08:7.38:8.14:13.05:27.41:39.02; the corresponding molar proportions for polysaccharide obtained from 70% ethanol solvent were 1:1.64:1.65:4.52:13.96:9.13:26.30:10.52:18.00; fucose and galacturonic acid were not found in the polysaccharide precipitated from 90% ethanol solvent, and mannose, xylose, glucuronic acid, glucose, rhamnose, galactose and arabinose were determined with molar proportion of 1:0.87:1.77:2.78:1.69:2.58:2.49. Quantitative recoveries of the component monosaccharides in the polysaccharide were in the range of 93.3-105.1% and the relative standard deviation (RSD) values fell within 3.4-6.3%, respectively. The results demonstrated that the proposed CZE method was precise and sensitive for the analysis of the composition of polysaccharide.; A rapid and sensitive method was optimized and validated for the separation and quantification of derivatized monosaccharides in polysaccharide from Potentilla anserine L. using 1-naphthyl-3-methyl-5-pyrazolone (NMP) as precolumn derivatization reagent by capillary zone electrophoresis (CZE). On the basis of the optimum conditions, nine NMP-derivatized monosaccharides achieve baseline resolution within 16 min. The developed method has been successfully applied to analyze component monosaccharides of three Potentilla anserine L. samples, which were obtained by gradational precipitation with 50, 70, and 90% aqueous ethanol, respectively. The polysaccharide precipitated from 50% ethanol solvent was composed of fucose, mannose, xylose, glucuronic acid, glucose, rhamnose, galacturonic acid, galactose, arabinose in molar proportion of 1:1.65:1.99:5.08:7.38:8.14:13.05:27.41:39.02; the corresponding molar proportions for polysaccharide obtained from 70% ethanol solvent were 1:1.64:1.65:4.52:13.96:9.13:26.30:10.52:18.00; fucose and galacturonic acid were not found in the polysaccharide precipitated from 90% ethanol solvent, and mannose, xylose, glucuronic acid, glucose, rhamnose, galactose and arabinose were determined with molar proportion of 1:0.87:1.77:2.78:1.69:2.58:2.49. Quantitative recoveries of the component monosaccharides in the polysaccharide were in the range of 93.3-105.1% and the relative standard deviation (RSD) values fell within 3.4-6.3%, respectively. The results demonstrated that the proposed CZE method was precise and sensitive for the analysis of the composition of polysaccharide.
关键词PotentiL.a Anserine L. Polysaccharide Monosaccharide Analytical Method
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]ANTIOXIDANT ACTIVITIES ; GREEN TEA ; MACROPHAGES ; CULTURE
收录类别SCI
语种英语
WOS研究方向Food Science & Technology
WOS类目Food Science & Technology
WOS记录号WOS:000274385400004
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1736
专题中国科学院西北高原生物研究所
作者单位1.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Coll Chem Sci, Qufu 273165, Peoples R China
2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Xia, Lian,Sun, Zhi Wei,Li, Guo Liang,et al. A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2010,230(5):715-722.
APA Xia, Lian,Sun, Zhi Wei,Li, Guo Liang,Suo, You Rui,&You, Jin Mao.(2010).A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,230(5),715-722.
MLA Xia, Lian,et al."A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 230.5(2010):715-722.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
A sensitive analytic(284KB) 开放获取--浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xia, Lian]的文章
[Sun, Zhi Wei]的文章
[Li, Guo Liang]的文章
百度学术
百度学术中相似的文章
[Xia, Lian]的文章
[Sun, Zhi Wei]的文章
[Li, Guo Liang]的文章
必应学术
必应学术中相似的文章
[Xia, Lian]的文章
[Sun, Zhi Wei]的文章
[Li, Guo Liang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: A sensitive analytical method for the component monosaccharides of the polysaccharides from a Tibetan herb Potentilla anserine L. by capillary zone electrophoresis with UV detector.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。