中国科学院西北高原生物研究所机构知识库

QQ客服 官方微博 反馈留言

	Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification
	Suo, Yourui1; Wang, Lingyun1,2
	2010-03-01
发表期刊	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
ISSN	1438-7697
卷号	112期号:3页码:390-399
文章类型	Article
摘要	The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre-column derivatization was developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with gradient elution. Identification of fatty acid derivatives was carried out by on-line APCI/MS in positive-ion mode. Excellent quantitative linear responses of the 39 fatty acids were observed in the range of 0.014 to 14 mu mol/L with correlation coefficients higher than 0.9992. Limits of detection were in the range of 0.32-3.7 nmol/L (S/N = 3). The fatty acids in Nitraria tangutorum seed lipid with or without saponification extracted by the four different methods were determined and compared. The results indicated that the mass percentage of unsaturated fatty acids (mainly oleic acid, linoleic acid and linolenic acid) in Nitraria tangutorum seed lipid was up to 79%, and the best method was supercritical carbon dioxide extraction.; The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre-column derivatization was developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C(8) column in conjunction with gradient elution. Identification of fatty acid derivatives was carried out by on-line APCI/MS in positive-ion mode. Excellent quantitative linear responses of the 39 fatty acids were observed in the range of 0.014 to 14 mu mol/L with correlation coefficients higher than 0.9992. Limits of detection were in the range of 0.32-3.7 nmol/L (S/N = 3). The fatty acids in Nitraria tangutorum seed lipid with or without saponification extracted by the four different methods were determined and compared. The results indicated that the mass percentage of unsaturated fatty acids (mainly oleic acid, linoleic acid and linolenic acid) in Nitraria tangutorum seed lipid was up to 79%, and the best method was supercritical carbon dioxide extraction.
关键词	Extraction Fatty Acids Fluorescence Detection Hplc Nitraria Tangutorum Bobr.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLVENT-EXTRACTION ; MASS-SPECTROMETRY ; BRYOPHYTE PLANTS ; CARBOXYLIC-ACIDS ; SIMULATION ; SOIL
收录类别	SCI
语种	英语
WOS研究方向	Food Science & Technology ; Nutrition & Dietetics
WOS类目	Food Science & Technology ; Nutrition & Dietetics
WOS记录号	WOS:000276280400012
引用统计	被引频次：19[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1740
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Suo, Yourui,Wang, Lingyun. Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2010,112(3):390-399.
APA	Suo, Yourui,&Wang, Lingyun.(2010).Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,112(3),390-399.
MLA	Suo, Yourui,et al."Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identification".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 112.3(2010):390-399.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
Extraction of Nitrar（343KB）			开放获取	--	浏览 下载

个性服务

推荐该条目

保存到收藏夹

查看访问统计

导出为Endnote文件

谷歌学术

谷歌学术中相似的文章

[Suo, Yourui]的文章

[Wang, Lingyun]的文章

百度学术

百度学术中相似的文章

[Suo, Yourui]的文章

[Wang, Lingyun]的文章

必应学术

必应学术中相似的文章

[Suo, Yourui]的文章

[Wang, Lingyun]的文章

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。