The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development

NWIPB OpenIR

	The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development
	Yang, Qi-En ; Ozawa, Manabu ; Zhang, Kun ; Johnson, Sally E. ; Ealy, Alan D.
	2016
发表期刊	REPRODUCTION FERTILITY AND DEVELOPMENT
摘要	Protein kinase C (PKC) delta (PRKCD) is a member of the novel PKC subfamily that regulates gene expression in bovine trophoblast cells. Additional functions for PRKCD in early embryonic development in cattle have not been fully explored. The objectives of this study were to describe the expression profile of PRKCD mRNA in bovine embryos and to examine its biological roles during bovine embryo development. Both PRKCD mRNA and protein are present throughout early embryo development and increases in mRNA abundance are evident at morula and blastocyst stages. Phosphorylation patterns are consistent with detection of enzymatically active PRKCD in bovine embryos. Exposure to a pharmacological inhibitor (rottlerin) during early embryonic development prevented development beyond the eight- to 16-cell stage. Treatment at or after the 16-cell stage reduced blastocyst development rates, total blastomere numbers and inner cell mass-to-trophoblast cell ratio. Exposure to the inhibitor also decreased basal interferon tau (IFNT) transcript abundance and abolished fibroblast growth factor-2 induction of IFNT expression. Furthermore, trophoblast adhesion and proliferation was compromised in hatched blastocysts. These observations provide novel insights into PRKCD mRNA expression profiles in bovine embryos and provide evidence for PRKCD-dependent regulation of embryonic development, gene expression and post-hatching events.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/17440
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,et al. The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development[J]. REPRODUCTION FERTILITY AND DEVELOPMENT,2016.
APA	Yang, Qi-En,Ozawa, Manabu,Zhang, Kun,Johnson, Sally E.,&Ealy, Alan D..(2016).The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development.REPRODUCTION FERTILITY AND DEVELOPMENT.
MLA	Yang, Qi-En,et al."The requirement for protein kinase C delta (PRKCD) during preimplantation bovine embryo development".REPRODUCTION FERTILITY AND DEVELOPMENT (2016).