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Cloning and characterization of a y-type inactive HMW glutenin subunit gene from Triticum durum cultivar youmangbingmai
Xiang, Weiwei1,2; Liu, Baolong1,2; Zhang, Huaigang1
2010-02-15
发表期刊AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN1684-5315
卷号9期号:7页码:967-971
文章类型Article
摘要The high molecular weight glutenin subunits (HMW-GS) are key factors of the breadmaking quality of common wheat flour. In the present study, one unexpressed 1By gene from Triticum durum cultivar youmangbingmai was cloned and characterized. The results indicated that the silenced 1By gene in youmangbingmai showed the highest homology to that of previously published 1By8, suggesting that the unexpressed 1By may have originated from 1By8. However, compared to expressed 1By8, the silenced 1By in youmangbingmai had a deletion of 247bp at 5' segment. The deletion resulted in the intact of DNA sequences responsible for signal peptide and N-terminal domains. The deletion also resulted in frameshift mutation in C-terminal domains and central region. Therefore, 1By gene in youmangbingmai had no the typical structure as did normal HMW-GS genes and thus this gene can be not translated into normal HWM-GS. This may be the molecular mechanism of silence for 1By gene in youmangbingmai. This molecular mechanism is different from those previously reported. Previous studies indicated that the insertion of transposon elements or the presence of premature stop codon is the molecular mechanisms responsible for silence of HWM-GS genes. Moreover, this deletion of 247 nucleotides in YMBM produced a new reading frame. This reading frame may produce a new protein, which is different from HWM-GS.; The high molecular weight glutenin subunits (HMW-GS) are key factors of the breadmaking quality of common wheat flour. In the present study, one unexpressed 1By gene from Triticum durum cultivar youmangbingmai was cloned and characterized. The results indicated that the silenced 1By gene in youmangbingmai showed the highest homology to that of previously published 1By8, suggesting that the unexpressed 1By may have originated from 1By8. However, compared to expressed 1By8, the silenced 1By in youmangbingmai had a deletion of 247bp at 5' segment. The deletion resulted in the intact of DNA sequences responsible for signal peptide and N-terminal domains. The deletion also resulted in frameshift mutation in C-terminal domains and central region. Therefore, 1By gene in youmangbingmai had no the typical structure as did normal HMW-GS genes and thus this gene can be not translated into normal HWM-GS. This may be the molecular mechanism of silence for 1By gene in youmangbingmai. This molecular mechanism is different from those previously reported. Previous studies indicated that the insertion of transposon elements or the presence of premature stop codon is the molecular mechanisms responsible for silence of HWM-GS genes. Moreover, this deletion of 247 nucleotides in YMBM produced a new reading frame. This reading frame may produce a new protein, which is different from HWM-GS.
关键词1by Gene Silence Glu-b1 Locus High Molecular Weight Glutenin Subunit
WOS标题词Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]MOLECULAR-WEIGHT SUBUNIT ; STORAGE PROTEINS ; WHEAT ; ALLELE ; QUALITY
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000275382200005
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1744
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Inst Plateau Biol, Xining 810001, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
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Xiang, Weiwei,Liu, Baolong,Zhang, Huaigang. Cloning and characterization of a y-type inactive HMW glutenin subunit gene from Triticum durum cultivar youmangbingmai[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2010,9(7):967-971.
APA Xiang, Weiwei,Liu, Baolong,&Zhang, Huaigang.(2010).Cloning and characterization of a y-type inactive HMW glutenin subunit gene from Triticum durum cultivar youmangbingmai.AFRICAN JOURNAL OF BIOTECHNOLOGY,9(7),967-971.
MLA Xiang, Weiwei,et al."Cloning and characterization of a y-type inactive HMW glutenin subunit gene from Triticum durum cultivar youmangbingmai".AFRICAN JOURNAL OF BIOTECHNOLOGY 9.7(2010):967-971.
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