GENETIC VARIATION AND MOLECULAR EVOLUTION OF ENDANGERED KOZLOV'S PIKA (OCHOTONA KOSLOWI BUCHNER) BASED ON MITOCHONDRIAL CYTOCHROME B GENE

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	GENETIC VARIATION AND MOLECULAR EVOLUTION OF ENDANGERED KOZLOV'S PIKA (OCHOTONA KOSLOWI BUCHNER) BASED ON MITOCHONDRIAL CYTOCHROME B GENE
	Lin, Gonghua 1,2; Ci, Haixin 1,2; Thirgood, Simon J.3; Zhang, Tongzuo 1; Su, Jianping 1
	2010
发表期刊	POLISH JOURNAL OF ECOLOGY
ISSN	1505-2249
卷号	58 期号:3 页码:563-568
文章类型	Article
摘要	Kozlov's pika is a rare and endangered lagomorph species with a limited distribution in the southern Kunlun Mountains in western China. Because of its endangered status, Kozlov's pika is considered a priority species for research and conservation action. Genetic variation and molecular evolution of the Kozlov's pika were studied based on a total of 14 individuals from four locations along the eastern boundary between Xinjiang and Tibet province (35.20-36.48 degrees N, 86.08-83.04 degrees E) on extremely high elevation (usually over 4800 m a.s.l.). The density of local populations was about 3-4 per ha, living in a typical alpine desert grassland habitat. The complete mitochondrial cytochrome b (cytb) gene was amplified and sequenced. Based on the cytb gene sequences the genetic variation and molecular evolution were analyzed. Unexpected high haplotype diversity (0.956 +/- 0.045) but low nucleotide diversity (0.00537 +/- 0.00126) was found, indicating past demographic expansion. Significant partitioning of variance (P <0.01) among populations (46.7%), and within populations (53.3%), indicating low level of genetic differentiations among local populations. Our results gave an optimistic survival status of Kozlov's pika at the genetic level. Bayes Empirical Bayes analysis with model M2a and M8 detected three positively selected amino acid sites at the significance level of 0.05. The mutant types with either or both of the mutations aspartic acid to asparagine and glutamic acid to lysine had higher isoelectric point values. We suggested these mutant types might have biological significance to help individuals to adapt to the extremely high elevation habitats.; Kozlov's pika is a rare and endangered lagomorph species with a limited distribution in the southern Kunlun Mountains in western China. Because of its endangered status, Kozlov's pika is considered a priority species for research and conservation action. Genetic variation and molecular evolution of the Kozlov's pika were studied based on a total of 14 individuals from four locations along the eastern boundary between Xinjiang and Tibet province (35.20-36.48 degrees N, 86.08-83.04 degrees E) on extremely high elevation (usually over 4800 m a.s.l.). The density of local populations was about 3-4 per ha, living in a typical alpine desert grassland habitat. The complete mitochondrial cytochrome b (cytb) gene was amplified and sequenced. Based on the cytb gene sequences the genetic variation and molecular evolution were analyzed. Unexpected high haplotype diversity (0.956 +/- 0.045) but low nucleotide diversity (0.00537 +/- 0.00126) was found, indicating past demographic expansion. Significant partitioning of variance (P <0.01) among populations (46.7%), and within populations (53.3%), indicating low level of genetic differentiations among local populations. Our results gave an optimistic survival status of Kozlov's pika at the genetic level. Bayes Empirical Bayes analysis with model M2a and M8 detected three positively selected amino acid sites at the significance level of 0.05. The mutant types with either or both of the mutations aspartic acid to asparagine and glutamic acid to lysine had higher isoelectric point values. We suggested these mutant types might have biological significance to help individuals to adapt to the extremely high elevation habitats.
关键词	Kozlov's Pika Genetic Diversity Genetic Structure Molecular Evolution
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SOFTWARE ; DNA
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Ecology
WOS记录号	WOS:000281936100015
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1770
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Macaulay Inst, Aberdeen AB15 8QH, Scotland
推荐引用方式 GB/T 7714	Lin, Gonghua,Ci, Haixin,Thirgood, Simon J.,et al. GENETIC VARIATION AND MOLECULAR EVOLUTION OF ENDANGERED KOZLOV'S PIKA (OCHOTONA KOSLOWI BUCHNER) BASED ON MITOCHONDRIAL CYTOCHROME B GENE[J]. POLISH JOURNAL OF ECOLOGY,2010,58(3):563-568.
APA	Lin, Gonghua,Ci, Haixin,Thirgood, Simon J.,Zhang, Tongzuo,&Su, Jianping.(2010).GENETIC VARIATION AND MOLECULAR EVOLUTION OF ENDANGERED KOZLOV'S PIKA (OCHOTONA KOSLOWI BUCHNER) BASED ON MITOCHONDRIAL CYTOCHROME B GENE.POLISH JOURNAL OF ECOLOGY,58(3),563-568.
MLA	Lin, Gonghua,et al."GENETIC VARIATION AND MOLECULAR EVOLUTION OF ENDANGERED KOZLOV'S PIKA (OCHOTONA KOSLOWI BUCHNER) BASED ON MITOCHONDRIAL CYTOCHROME B GENE".POLISH JOURNAL OF ECOLOGY 58.3(2010):563-568.