Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification

NWIPB OpenIR

	Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification
	Fu Yan-Yan 1; Li Xiao-Yan 1; Sun Zhi-Wei 2,3; Qin Xue-Qin 1; Xia Lian 1,2,3; Suo You-Rui 2; Li Yu-Lin 2; You Jin-Mao 1,2
	2010
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	38 期号:1 页码:8-14
文章类型	Article
摘要	With comparison of three different methods for the marking of amines compound, an optimal derivatization method was selected. 5-(2-Hydroxyethyl) benzoacridine (HBA) reacts with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 2-(benzoacridin) ethyl-imidazole-1-carboxylate(BAEIC). BAEIC, which is dual-sensitive probe, reacts preferably with amino compounds at 80 degrees C in the presence of 4-dimethylaminopyridine (DMAP) catalyst in N, N-dimethylformamide (DMF) solvent to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) of 280 nm and an emission maximum at lambda(em) of 510 nm. BAEIC-amine derivatives simultaneously exhibited high. ionization potential with percent ionization (changing from 5.62% to 58.08% in aqueous acetonitrile and from 2.14% to 56.58% in aqueous methanol. Derivatives were not only sensitive to fluorescence but also to MS ionizable potential. The fluorescence detection limits(S/N = 3) were 0.12 - 0. 59 mu g/L. The online APCI-MS detection limits were 1.9-14 mu g/L (S/N = 5); With comparison of three different methods for the marking of amines compound, an optimal derivatization method was selected. 5-(2-Hydroxyethyl) benzoacridine (HBA) reacts with coupling agent N, N'-carbonyldiimidazole (CDI) to form an activated amide intermediate 2-(benzoacridin) ethyl-imidazole-1-carboxylate(BAEIC). BAEIC, which is dual-sensitive probe, reacts preferably with amino compounds at 80 degrees C in the presence of 4-dimethylaminopyridine (DMAP) catalyst in N, N-dimethylformamide (DMF) solvent to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda(ex) of 280 nm and an emission maximum at lambda(em) of 510 nm. BAEIC-amine derivatives simultaneously exhibited high. ionization potential with percent ionization (changing from 5.62% to 58.08% in aqueous acetonitrile and from 2.14% to 56.58% in aqueous methanol. Derivatives were not only sensitive to fluorescence but also to MS ionizable potential. The fluorescence detection limits(S/N = 3) were 0.12 - 0. 59 mu g/L. The online APCI-MS detection limits were 1.9-14 mu g/L (S/N = 5)
关键词	High Performance Liquid Chromatography/mass Spectrometry Aliphatic Amines 5-(2-hydroxyethyl) Benzoacridine 2-(Benzoacridin) Ethyl-imidazole-1-carboxylate
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PRECOLUMN DERIVATIZATION AGENT ; LIQUID-CHROMATOGRAPHY ; FLUOROMETRIC DETECTION ; WASTE-WATER ; ACIDS ; CHLORIDE ; HPLC
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000274371500002
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1772
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Fu Yan-Yan,Li Xiao-Yan,Sun Zhi-Wei,et al. Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2010,38(1):8-14.
APA	Fu Yan-Yan.,Li Xiao-Yan.,Sun Zhi-Wei.,Qin Xue-Qin.,Xia Lian.,...&You Jin-Mao.(2010).Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,38(1),8-14.
MLA	Fu Yan-Yan,et al."Dual-Sensitive Probe of 2-(Benzoacridin) ethyl-imidazole-1-carboxylate for Determination of Aliphatic Amine with Fluorescence and Online Atmospheric Pressure Chemical Ionization Mass Spectrometry Identification".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 38.1(2010):8-14.