Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses

NWIPB OpenIR

	Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses
	Jiang, Yuanzhong ; Duan, Yanjiao ; Yin, Jia ; Ye, Shenglong ; Zhu, Jingru ; Zhang, Faqi ; Lu, Wanxiang ; Fan, Di ; Luo, Keming ; Luo, KM (reprint author), Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing Key Lab Transgen Plant & Safety Control, Chongqing 400715, Peoples R China.
	2014-12-01
发表期刊	JOURNAL OF EXPERIMENTAL BOTANY
摘要	WRKY proteins are a large family of regulators involved in various developmental and physiological processes, especially in coping with diverse biotic and abiotic stresses. In this study, 100 putative PtrWRKY genes encoded the proteins contained in the complete WRKY domain in Populus. Phylogenetic analysis revealed that the members of this superfamily among poplar, Arabidopsis, and other species were divided into three groups with several subgroups based on the structures of the WRKY protein sequences. Various cis-acting elements related to stress and defence responses were found in the promoter regions of PtrWRKY genes by promoter analysis. High-throughput transcriptomic analyses identified that 61 of the PtrWRKY genes were induced by biotic and abiotic treatments, such as Marssonina brunnea, salicylic acid (SA), methyl jasmonate (MeJA), wounding, cold, and salinity. Among these PtrWRKY genes, transcripts of 46 selected genes were observed in different tissues, including roots, stems, and leaves. Quantitative RT-PCR analysis further confirmed the induced expression of 18 PtrWRKY genes by one or more stress treatments. The overexpression of an SA-inducible gene, PtrWRKY89, accelerated expression of PR protein genes and improved resistance to pathogens in transgenic poplar, suggesting that PtrWRKY89 is a regulator of an SA-dependent defence-signalling pathway in poplar. Taken together, our results provided signi.cant information for improving the resistance and stress tolerance of woody plants.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/17757
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jiang, Yuanzhong,Duan, Yanjiao,Yin, Jia,et al. Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses[J]. JOURNAL OF EXPERIMENTAL BOTANY,2014.
APA	Jiang, Yuanzhong.,Duan, Yanjiao.,Yin, Jia.,Ye, Shenglong.,Zhu, Jingru.,...&Luo, KM .(2014).Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses.JOURNAL OF EXPERIMENTAL BOTANY.
MLA	Jiang, Yuanzhong,et al."Genome-wide identification and characterization of the Populus WRKY transcription factor family and analysis of their expression in response to biotic and abiotic stresses".JOURNAL OF EXPERIMENTAL BOTANY (2014).