DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT

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	DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT
	Sun, Zhiwei 1,3; Xia, Lian 1,2,3; Suo, Yourui 1; You, Jinmao 1,2
	2010
发表期刊	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN	1082-6076
卷号	33 期号:3 页码:390-404
文章类型	Article
摘要	A novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (DBCPC-Cl) has been designed and synthesized for amine labeling in HPLC. Using 12 aliphatic amines as the models, the derivatization conditions were optimized. In 0.2mol/L borate buffer (pH 9.0), amines reacted with DBCPC-Cl at 30 degrees C to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by HPLC. The separation of these amine derivatives was achieved with a C8 column and gradient elution by using 30% acetonitrile (containing 20mmol/L formic acid) and 100% acetonitrile, and online APCI/MS identification of the derivatives was carried out in positive-ion mode. The fluorescence responses for DBCPC-derivatives were higher relative to those obtained using previously reported reagents. With fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). The relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. The mean recoveries ranged from 86.9 to 104.7%. Excellent linear responses were observed with coefficients >0.9991.; A novel fluorescent probe, 1-[1,2,5,6-dibenzocarbazol-9-yl]propan-2-yl chloroformate (DBCPC-Cl) has been designed and synthesized for amine labeling in HPLC. Using 12 aliphatic amines as the models, the derivatization conditions were optimized. In 0.2mol/L borate buffer (pH 9.0), amines reacted with DBCPC-Cl at 30 degrees C to form the derivatives in 3min, and the derivatives were stable enough to be efficiently analyzed by HPLC. The separation of these amine derivatives was achieved with a C8 column and gradient elution by using 30% acetonitrile (containing 20mmol/L formic acid) and 100% acetonitrile, and online APCI/MS identification of the derivatives was carried out in positive-ion mode. The fluorescence responses for DBCPC-derivatives were higher relative to those obtained using previously reported reagents. With fluorescence detection at an emission wavelength of 390nm and an excitation wavelength of 300nm, the detection limits of aliphatic amines were 0.3 - 5.1fmol (at a signal-to-noise ratio of 3:1). The relative standard deviations for within-day determination (n=12) were 0.051-0.079% for retention time and 0.84-1.12% for peak area for the tested aliphatic amines. The mean intra- and inter-assay precision for all amines levels were <2.52% and 3.74%, respectively. The mean recoveries ranged from 86.9 to 104.7%. Excellent linear responses were observed with coefficients >0.9991.
关键词	Aliphatic Amines 1-[1 Derivatization 2 Fluorescent Probe 5 Hplc 6-dibenzocarbazol-9-yl]Propan-2-yl Chloroformate (Dbcpc-cl)
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRECOLUMN DERIVATIZATION ; BIOGENIC-AMINES ; FLUORESCENCE DETECTION ; ACIDS ; DERIVATIVES ; SAMPLES
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000274174400008
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1778
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Qufu Normal Univ, Key Lab Life Organ Anal, Coll Chem Sci, Qufu, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhiwei,Xia, Lian,Suo, Yourui,et al. DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(3):390-404.
APA	Sun, Zhiwei,Xia, Lian,Suo, Yourui,&You, Jinmao.(2010).DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(3),390-404.
MLA	Sun, Zhiwei,et al."DETERMINATION AND IDENTIFICATION OF ALIPHATIC AMINES FROM ENVIRONMENTAL WATER WITH HPLC-FLD AND APCI/MS USING 1-[1,2,5,6-DIBENZOCARBAZOL-9-YL]PROPAN-2-YL CHLOROFORMATE (DBCPC-CL) AS NOVEL LABELING REAGENT".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.3(2010):390-404.