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HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L
Song, Cuihua3; Sun, Zhiwei1,2; Xia, Lian1,2,3; Suo, Yourui1; You, Jinmao1,3
2010
发表期刊JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN1082-6076
卷号33期号:6页码:859-874
文章类型Article
摘要A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined.; A sensitive HPLC method for the determination of fatty acids using 2-(2-(pyren-1-yl)-1H-benzo[d]imidazol-1-yl) ethyl-p-toluenesulfonate (PBIOTs) as a novel labeling reagent with fluorescence detection has been developed. PBIOTs could easily and quickly label fatty acids in the presence of the K2CO3 catalyst at 90 degrees C for 30min in N,N-dimethylformamide solvent. Fatty acids derivatives were separated on a reversed phase Eclipse XDB-C8 column by HPLC in conjunction with gradient elution. The corresponding derivatives were identified by post-column APCI-MS in positive ion detection mode. PBIOTs-fatty acid derivatives gave an intense molecular ion peak at m/z [M+H]+; with MS/MS analysis, the collision induced dissociation spectra of m/z [M+H]+ produced the specific fragment ions at m/z [M+H-319]+ and m/z 319.0 (here, m/z 319 is the core structural moiety of the PBIOTs molecule). The fluorescence excitation and emission wavelengths of the derivatives were ex=350nm and em=402nm, respectively. Linear correlation coefficients for all fatty acid derivatives were epsilon 0.9985. Detection limits for the labeled fatty acids, at a signal-to-noise ratio of 3:1, were in the range of 10.32- 44.28fmol. From the extracts of Stellera chamaejasma L, free fatty acids were sensitively determined.
关键词2-(2-(Pyren-1-yl)-1h-benzo[d]Imidazol-1-yl) Ethyl-p-toluenesulfonate (Pbiots) Atmospheric Pressure Chemical Ionization Mass Spectrometer (Apci-ms) Fatty Acids (Fas) Fluorescence Detection (Fld) High Performance Liquid Chromatography (Hplc) Stellera Chamaejasma l
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; MASS-SPECTROMETRIC IDENTIFICATION ; FLUORESCENCE DETECTION ; CARBOXYLIC-ACIDS ; MS ; REAGENT ; PLANTS
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000277574500013
引用统计
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/1782
专题中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, NW Plateau Inst Biol, Xining, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
3.Qufu Normal Univ, Key Lab Life Organ Anal Shandong Prov, Qufu, Peoples R China
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GB/T 7714
Song, Cuihua,Sun, Zhiwei,Xia, Lian,et al. HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(6):859-874.
APA Song, Cuihua,Sun, Zhiwei,Xia, Lian,Suo, Yourui,&You, Jinmao.(2010).HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(6),859-874.
MLA Song, Cuihua,et al."HPLC-FLUORIMETRIC METHOD FOR ANALYSIS OF FREE FATTY ACIDS IN STELLERA CHAMAEJASMA L".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.6(2010):859-874.
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