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Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS
Chen, Tao ; Li, Hongmei ; Tan, Liang ; Li, Yulin
2017
发表期刊SEPARATION SCIENCE AND TECHNOLOGY
摘要A minor lignan, 4'-demethyldeoxypodophyllotoxin, was isolated from Sinopodophyllum emodi. First, a crude extract of S. emodi was analyzed by high performance liquid chromatography-mass spectrometry (HPLC-MS) to confirm the presence of the target compound. Then, the target lignan was successfully enriched in the crude extract precipitate by medium-pressure LC (MPLC) with high recovery (85%). Finally, an efficient separation was achieved by high-speed counter-current chromatography (HSCCC) using a two-phase solvent system of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v/v/v). These results clearly demonstrate that the combination of HPLC-MS, MPLC, and HSCCC could be a powerful technique for the rapid screening, enrichment, and separation of minor compounds from complex natural products.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/17875
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Chen, Tao,Li, Hongmei,Tan, Liang,et al. Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS[J]. SEPARATION SCIENCE AND TECHNOLOGY,2017.
APA Chen, Tao,Li, Hongmei,Tan, Liang,&Li, Yulin.(2017).Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS.SEPARATION SCIENCE AND TECHNOLOGY.
MLA Chen, Tao,et al."Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS".SEPARATION SCIENCE AND TECHNOLOGY (2017).
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