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High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans
Dou, Quan-Wen1; Chen, Zhi-Guo1; Liu, Yong-An1; Tsujimoto, Hisashi2
2009-12-01
Source PublicationBREEDING SCIENCE
ISSN1344-7610
Volume59Issue:5Pages:651-656
SubtypeArticle
AbstractElymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed.; Elymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed.
KeywordPerennial Grass Forage Elymus Nutans Karyotype Variation Fish Gish
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS KeywordIN-SITU HYBRIDIZATION ; REPETITIVE SEQUENCES ; AFA-FAMILY ; TRITICEAE ; POACEAE ; IDENTIFICATION ; GENOME ; BARLEY ; LEYMUS ; WHEAT
Indexed BySCI ; ISTP
Language英语
WOS Research AreaAgriculture ; Plant Sciences
WOS SubjectAgronomy ; Plant Sciences
WOS IDWOS:000274757200026
Citation statistics
Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/1788
Collection中国科学院西北高原生物研究所
Affiliation1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China
2.Tottori Univ, Fac Agr, Tottori 6808550, Japan
Recommended Citation
GB/T 7714
Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,et al. High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J]. BREEDING SCIENCE,2009,59(5):651-656.
APA Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,&Tsujimoto, Hisashi.(2009).High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans.BREEDING SCIENCE,59(5),651-656.
MLA Dou, Quan-Wen,et al."High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans".BREEDING SCIENCE 59.5(2009):651-656.
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