High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans

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	High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans
	Dou, Quan-Wen 1; Chen, Zhi-Guo 1; Liu, Yong-An 1; Tsujimoto, Hisashi 2
	2009-12-01
发表期刊	BREEDING SCIENCE
ISSN	1344-7610
卷号	59 期号:5 页码:651-656
文章类型	Article
摘要	Elymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed.; Elymus nutans L. (StHY, 2n = 6x = 42) is extensively selected from the natural population and domesticated as perennial grass forage in the Qinghai-Tibet plateau in China due to its high tolerance to environmental stresses, such as cold and drought. Karyotyping was conducted in 12 randomly selected plants by sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH). GISH discriminated St, H and Y genomes, indicating that E. nutans has retained its ancestral genome, and large chromosomal rearrangements have not occurred. However, FISH using an AGG satellite and Afa-family repetitive sequences, revealed marked variation in the signalling patterns of most of the chromosomes. Many of the plants carried chromosomes with a specific pattern in the homozygous state, indicating that self-pollination or sib-crossing occurs in small populations during selection for domestication. In addition, several intergenomic translocations appeared, possibly caused by homoeologous chromosome recombination. The nature of the polymorphisms seen in the chromosomes of the domesticated Population of E. nutans is discussed.
关键词	Perennial Grass Forage Elymus Nutans Karyotype Variation Fish Gish
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	IN-SITU HYBRIDIZATION ; REPETITIVE SEQUENCES ; AFA-FAMILY ; TRITICEAE ; POACEAE ; IDENTIFICATION ; GENOME ; BARLEY ; LEYMUS ; WHEAT
收录类别	SCI ; ISTP
语种	英语
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agronomy ; Plant Sciences
WOS记录号	WOS:000274757200026
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1788
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Tottori Univ, Fac Agr, Tottori 6808550, Japan
推荐引用方式 GB/T 7714	Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,et al. High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans[J]. BREEDING SCIENCE,2009,59(5):651-656.
APA	Dou, Quan-Wen,Chen, Zhi-Guo,Liu, Yong-An,&Tsujimoto, Hisashi.(2009).High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans.BREEDING SCIENCE,59(5),651-656.
MLA	Dou, Quan-Wen,et al."High frequency of karyotype variation revealed by sequential FISH and GISH in plateau perennial grass forage Elymus nutans".BREEDING SCIENCE 59.5(2009):651-656.