Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.

NWIPB OpenIR

	Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.
	Wang, Liu-Yang 1,2,3; Kou, Yi-Xuan 1; Wu, Gui-Li 1; Wang, Yu-Jin 1,2
	2009-12-01
发表期刊	CONSERVATION GENETICS RESOURCES
ISSN	1877-7252
卷号	1 期号:1 页码:51-53
文章类型	Article
摘要	Here, we report development and characterization of 11 new polymorphic microsatellite loci isolated from the genome of Potentilla fruticosa L. (Rosaceae). Polymorphisms of each locus were screened in 12-15 individuals from geographically separated populations. The number of alleles per locus ranged from five to eleven. The observed and expected heterozygosity ranged from 0.22 to 0.35 and 0.38 to 0.53, respectively. These eleven loci were also surveyed for 11 individuals from its sister species, Potentilla glabra L. (Rosaceae). Ten of them were successfully amplified and allelic diversities were also highly polymorphic. Seven loci totally in both species exhibited significant departure from Hardy-Weinberg equilibrium probably due to excessive homozygosity and the population structure within samples. No linkage disequilibrium was detected among all paired loci comparisons. These results demonstrate that these microsatellite markers can be used to characterise the intra-and inter-specific genetic diversity, and will be useful to study the population genetics and speciation history of the two closely related shrubs.; Here, we report development and characterization of 11 new polymorphic microsatellite loci isolated from the genome of Potentilla fruticosa L. (Rosaceae). Polymorphisms of each locus were screened in 12-15 individuals from geographically separated populations. The number of alleles per locus ranged from five to eleven. The observed and expected heterozygosity ranged from 0.22 to 0.35 and 0.38 to 0.53, respectively. These eleven loci were also surveyed for 11 individuals from its sister species, Potentilla glabra L. (Rosaceae). Ten of them were successfully amplified and allelic diversities were also highly polymorphic. Seven loci totally in both species exhibited significant departure from Hardy-Weinberg equilibrium probably due to excessive homozygosity and the population structure within samples. No linkage disequilibrium was detected among all paired loci comparisons. These results demonstrate that these microsatellite markers can be used to characterise the intra-and inter-specific genetic diversity, and will be useful to study the population genetics and speciation history of the two closely related shrubs.
关键词	Potentilla Fruticosa Potentilla Glabra Microsatellite Genetic Diversity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
收录类别	SCI
语种	英语
WOS研究方向	Biodiversity & Conservation ; Genetics & Heredity
WOS类目	Biodiversity Conservation ; Genetics & Heredity
WOS记录号	WOS:000207922400013
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1802
专题	中国科学院西北高原生物研究所
作者单位	1.Lanzhou Univ, Inst Mol Ecol, Key Lab Arid & Grassland Ecol, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China 2.Chinese Acad Sci, NW Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Liu-Yang,Kou, Yi-Xuan,Wu, Gui-Li,et al. Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.[J]. CONSERVATION GENETICS RESOURCES,2009,1(1):51-53.
APA	Wang, Liu-Yang,Kou, Yi-Xuan,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L..CONSERVATION GENETICS RESOURCES,1(1),51-53.
MLA	Wang, Liu-Yang,et al."Development and characterization of novel microsatellite markers isolated from Potentilla fruticosa L. (Rosaceae), and cross-species amplification in its sister species-Potentilla glabra L.".CONSERVATION GENETICS RESOURCES 1.1(2009):51-53.