Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy

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	Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy
	Sun, Zhiwei 1,3; You, Jinmao 1,2; Xia, Lian 1,3; Suo, Yourui 1
	2009-10-01
发表期刊	CHROMATOGRAPHIA
ISSN	0009-5893
卷号	70 期号:7-8 页码:1055-1063
文章类型	Article
摘要	4-(1H-Phenanthro[9,10-d]imidazol-2-yl)benzoic acid (PIBA), a new amine-reactive fluorescent probe was synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines from environmetal water samples. Derivatization was successfully carried out by a condensation reaction using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride as the dehydrant. Studies on derivatization conditions indicated that the condensation reaction proceeded rapidly and smoothly in the presence of pyridine. Amines were labeled with PIBA to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda (ex) 260 nm and an emission maximum at lambda (em) 445 nm. Identification of derivatives was carried out by online APCI-MS-MS and showed an intense protonated molecular ion corresponding m/z [M + H](+) in the positive ion mode. Excellent linear responses were observed with coefficients of > 0.9996. Detection limits were 6.7-27 fmol (at a signal-to-noise ratio of 3). The mean intra- and inter-assay precision for all amine levels were < 2.79 and 3.85%, respectively. The established method for the determination of aliphatic amines from real water was satisfactory.; 4-(1H-Phenanthro[9,10-d]imidazol-2-yl)benzoic acid (PIBA), a new amine-reactive fluorescent probe was synthesized and used as a pre-column derivatizing reagent for the determination of aliphatic amines from environmetal water samples. Derivatization was successfully carried out by a condensation reaction using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride as the dehydrant. Studies on derivatization conditions indicated that the condensation reaction proceeded rapidly and smoothly in the presence of pyridine. Amines were labeled with PIBA to give the corresponding sensitively fluorescent derivatives with an excitation maximum at lambda (ex) 260 nm and an emission maximum at lambda (em) 445 nm. Identification of derivatives was carried out by online APCI-MS-MS and showed an intense protonated molecular ion corresponding m/z [M + H](+) in the positive ion mode. Excellent linear responses were observed with coefficients of > 0.9996. Detection limits were 6.7-27 fmol (at a signal-to-noise ratio of 3). The mean intra- and inter-assay precision for all amine levels were < 2.79 and 3.85%, respectively. The established method for the determination of aliphatic amines from real water was satisfactory.
关键词	Column Liquid Chromatographia Mass Spectrometry Pre-column Derivatization Fluorescence Detection Aliphatic Amines
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; SOLID-PHASE MICROEXTRACTION ; N-HYDROXYSUCCINIMIDE ESTER ; DERIVATIZING REAGENT ; BIOGENIC-AMINES ; WASTE-WATER ; 9-FLUORENYLMETHYL CHLOROFORMATE ; SPECTROMETRY ; SEPARATION ; CHLORIDE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000271069400006
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1820
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 2.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhiwei,You, Jinmao,Xia, Lian,et al. Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy[J]. CHROMATOGRAPHIA,2009,70(7-8):1055-1063.
APA	Sun, Zhiwei,You, Jinmao,Xia, Lian,&Suo, Yourui.(2009).Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy.CHROMATOGRAPHIA,70(7-8),1055-1063.
MLA	Sun, Zhiwei,et al."Determination and Identification of Primary Aliphatic Amines Using 4-(1H-Phenanthro[9,10-d]Imidazol-2-yl)Benzoic Acid as Novel Pre-Column Labeling Reagent by LC with Fluorescence Detection and Atmospheric Pressure Chemical Ionization Mass Spectroscopy".CHROMATOGRAPHIA 70.7-8(2009):1055-1063.