Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro

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	Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro
	Wang, Li 1,2,3; An, Lizhe 2; Hu, Yanping 1; Wei, Lixin 1; Li, Yi 1
	2009-06-03
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
ISSN	1684-5315
卷号	8 期号:11 页码:2513-2517
文章类型	Article
摘要	A procedure for in vitro shoot regeneration was formed from leaves derived from the field grown Swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. The best result was obtained in the 190-2 medium with 13.32 mu M 6-benzylaminopurine and 0.54 mu M alpha-naphthaleneacetic acid. The result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. The propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. The highest rate of shoots was 96.50% on the medium with 5.40 mu M alpha-naphthaleneacetic acid. Histological studies showed shoot regeneration took place by somatic embryogenesis.; A procedure for in vitro shoot regeneration was formed from leaves derived from the field grown Swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. The best result was obtained in the 190-2 medium with 13.32 mu M 6-benzylaminopurine and 0.54 mu M alpha-naphthaleneacetic acid. The result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. The propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. The highest rate of shoots was 96.50% on the medium with 5.40 mu M alpha-naphthaleneacetic acid. Histological studies showed shoot regeneration took place by somatic embryogenesis.
关键词	Swertia Chirata Adventitious Shoots Histology An Orthogonal Test Leaves
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	ENDANGERED MEDICINAL HERB ; SOMATIC EMBRYOGENESIS ; EXPLANTS ; MULTIPLICATION ; ORGANOGENESIS ; CULTURES ; CASSAVA ; PLANTS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000268830900018
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1858
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Inst Plateau Biol, Xining 810001, Peoples R China 2.Chinese Acad Sci, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou 730000, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Li,An, Lizhe,Hu, Yanping,et al. Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2009,8(11):2513-2517.
APA	Wang, Li,An, Lizhe,Hu, Yanping,Wei, Lixin,&Li, Yi.(2009).Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro.AFRICAN JOURNAL OF BIOTECHNOLOGY,8(11),2513-2517.
MLA	Wang, Li,et al."Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro".AFRICAN JOURNAL OF BIOTECHNOLOGY 8.11(2009):2513-2517.