Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent

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	Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent
	Yan Tao 1; Sun Xue-Jun 1; Zhao Huai-Xin 1; Sun Zhi-Wei 1; Liu Qin-Ze 1; Suo You-Rui 2; You Jin-Mao 1,2
	2009-06-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	37 期号:6 页码:788-794
文章类型	Article
摘要	On a reversed-phase Eclipse XDB C8 ( 150 mm x 4.6 mm, 5 mu m) column, 12 aliphatic amine derivatives were derivatized using 2-[2-(7H-dibenzo[a, g] carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase. Detection was carried out by the fluorescence and online post-column atmospheric pressure chemical ionization(APCI) in positive-ion mode. The maximum excitation and emission wavelengths were at 300 nm and 400 nm, respectively. Identification of aliphatic amine derivatives was carried out by the online post-column Ion trap/mass spectrometry with positive-ion detection mode of APCI-MS. A rapid, accurate method for the determination of free aliphatic amine extracted from soil was proposed. The established method exhibited excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0.9991. The detection limits( at signal-to-noise of 3:1) were 10.1-0.3 fmol.; On a reversed-phase Eclipse XDB C8 ( 150 mm x 4.6 mm, 5 mu m) column, 12 aliphatic amine derivatives were derivatized using 2-[2-(7H-dibenzo[a, g] carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase. Detection was carried out by the fluorescence and online post-column atmospheric pressure chemical ionization(APCI) in positive-ion mode. The maximum excitation and emission wavelengths were at 300 nm and 400 nm, respectively. Identification of aliphatic amine derivatives was carried out by the online post-column Ion trap/mass spectrometry with positive-ion detection mode of APCI-MS. A rapid, accurate method for the determination of free aliphatic amine extracted from soil was proposed. The established method exhibited excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0.9991. The detection limits( at signal-to-noise of 3:1) were 10.1-0.3 fmol.
关键词	High Performance Liquid Chromatography/ion-trap Mass Spectrometry Fluorescence Detection Precolumn Derivatization Aliphatic Amines 2-[2-(7h-dibenzo[a g] Carbazol-7-yl)-ethoxy] Ethyl Chloroformate
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; PRECOLUMN DERIVATIZATION ; 6-AMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE ; ORTHO-PHTHALDIALDEHYDE ; ACIDS ; HYDROLYSATE
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000267385100002
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1864
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Shandong Key Lab Life Organ Anal, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Yan Tao,Sun Xue-Jun,Zhao Huai-Xin,et al. Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(6):788-794.
APA	Yan Tao.,Sun Xue-Jun.,Zhao Huai-Xin.,Sun Zhi-Wei.,Liu Qin-Ze.,...&You Jin-Mao.(2009).Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(6),788-794.
MLA	Yan Tao,et al."Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.6(2009):788-794.