Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)

NWIPB OpenIR

	Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)
	Li, Zhong-Hu 1,2; Wen, Hong-Yan 3; Chen, Jian 1; Wu, Gui-Li 1; Wang, Yu-Jin 1,2
	2009-06-01
发表期刊	CONSERVATION GENETICS
ISSN	1566-0621
卷号	10 期号:3 页码:747-749
文章类型	Article
摘要	Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species. These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.; Codonopsis pilosula Nannf., as an important traditional Chinese medicinal plant species, has been used to treat fatigue, thirst and loss of appetite. In this study, we developed 10 new microsatellite loci primers from the genome of this species using the combined biotin capture method. The polymorphism of each locus was further assessed through 27 individuals from four geographically distant populations. The number of alleles per locus ranged from 6 to 11. The observed and expected heterozygosity ranged from 0.15 to 0.24 and 0.27 to 0.40, respectively. We further performed cross-priming tests of these loci in the other three congeneric species. These microsatellite markers will be useful for investigating genetic diversity within and between populations of these species.
关键词	Codonopsis Pilosula Nannf. Microsatellite Markers Genetic Diversity
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
收录类别	SCI
语种	英语
WOS研究方向	Biodiversity & Conservation ; Genetics & Heredity
WOS类目	Biodiversity Conservation ; Genetics & Heredity
WOS记录号	WOS:000265832000060
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1868
专题	中国科学院西北高原生物研究所
作者单位	1.Lanzhou Univ, Key Lab Arid & Grassland Ecol, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810008, Peoples R China 3.Management Off Innovat & Dev Biol Resources, Lijiang 674100, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,et al. Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)[J]. CONSERVATION GENETICS,2009,10(3):747-749.
APA	Li, Zhong-Hu,Wen, Hong-Yan,Chen, Jian,Wu, Gui-Li,&Wang, Yu-Jin.(2009).Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae).CONSERVATION GENETICS,10(3),747-749.
MLA	Li, Zhong-Hu,et al."Development of 10 polymorphic microsatellite loci primers for Codonopsis pilosula Nannf. (Campanulaceae)".CONSERVATION GENETICS 10.3(2009):747-749.