Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)

NWIPB OpenIR

	Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)
	Li, Ke-xin 1,2; Geng, Jia-ning 2,3; Yang, Jin 3; Zhang, Yan-ming 1; Hu, Song-nian 3
	2009-06-01
发表期刊	CONSERVATION GENETICS
ISSN	1566-0621
卷号	10 期号:3 页码:785-787
文章类型	Article
摘要	High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.; High quality jumping microsatellite libraries of the plateau pika (Ochotona curzoniae) were constructed; 231 out of the 288 clones contained microsatellite repeat motif and 120 pairs of primers were designed accordingly. Polymorphism was assessed for 48 individuals. Only 13 microsatellite loci were polymorphic with high polymorphism information content (PIC) ranging from 0.598 to 0.871, a condition probably resulting from individuals used for assessment being very closely related. This degree of polymorphism, however, is sufficient to conduct parentage analysis. Expected and observed heterozygosities ranged from 0.219 to 0.935 and 0.659 to 0.890, respectively. One locus was in linkage disequilibrium. This information provides efficient tools to allow future parentage studies.
关键词	Magnetic Beads Enrichment Microsatellite Parentage Analysis Plateau Pika Polymorphism
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	SOFTWARE
收录类别	SCI
语种	英语
WOS研究方向	Biodiversity & Conservation ; Genetics & Heredity
WOS类目	Biodiversity Conservation ; Genetics & Heredity
WOS记录号	WOS:000265832000069
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1872
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, Key Lab Adaptat & Evolut Plateau Biota, NW Plateau Inst Biol, Xining 810001, Qinghai, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Chinese Acad Sci, Key Lab Genome Informat & Sci, Beijing Inst Genom, Beijing 100029, Peoples R China
推荐引用方式 GB/T 7714	Li, Ke-xin,Geng, Jia-ning,Yang, Jin,et al. Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)[J]. CONSERVATION GENETICS,2009,10(3):785-787.
APA	Li, Ke-xin,Geng, Jia-ning,Yang, Jin,Zhang, Yan-ming,&Hu, Song-nian.(2009).Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae).CONSERVATION GENETICS,10(3),785-787.
MLA	Li, Ke-xin,et al."Isolation and characterization of 13 microsatellite loci in the plateau pika (Ochotona curzoniae)".CONSERVATION GENETICS 10.3(2009):785-787.