Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS

NWIPB OpenIR

	Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS
	Zhao Huai-Xin 1; Sun Xue-Jun 1; Sun Zhi-Wei 2,3; Yan Tao 1; Zhang Zai-Qing 1; Liu Qin-Ze 1; Suo You-Rui 2; Li Yu-Lin 2; You Jin-Mao 1,2
	2009-04-10
发表期刊	CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN	0251-0790
卷号	30 期号:4 页码:675-681
文章类型	Article
摘要	On a reversed-phase Hypersil BDS C(18) (4.6 mm x 200 mm, 10 mu m i.d.) column, 12 aliphatic amines were derivatized using 10-ethyl-9-oxo-9,10-dihydroacriditie-2-sulfonyl chloride(EASC) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase within 20 min. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 270 nm/430 nm. Comparing with the widely used Dansyl-Cl, EASC has stronger photoluminescence characteristics(ultraviolet and fluorescence) : UV(EASC)/UV(Dansyl-Cl) = 3.2 (UV: ultraviolet absorption intensity); I(EASC/I(Dansyl-Cl) = 30.0: 1-105. 4:1 (I: relative fluorescence intensity); fluorescence quantum yield: Phi(f)(EASC)/Phi(f)(Dansyl-Cl) = 43.0:1. Fast and accurate quantitative analysis of aliphatic amines with fluorescence and corresponding post-column ion trap mass spectrometry(LC-APCI-MS) were obtained. EASC-amines exhibited stronger mass spectrometric sensitivity relative to that of dansyl-amine derivatives: MS(EASC)/MS(Dansyl-Cl) = 1. 6: 1-6. 2: 1 times(MS: relative intensity of ion current). The established method for the determination of aliphatic amines in environmental water samples exhibited good reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients >0.9995. The detection limits(at signal-to-noise of 3: 1) were at levels of 4.0-12.7 fmol.; On a reversed-phase Hypersil BDS C(18) (4.6 mm x 200 mm, 10 mu m i.d.) column, 12 aliphatic amines were derivatized using 10-ethyl-9-oxo-9,10-dihydroacriditie-2-sulfonyl chloride(EASC) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase within 20 min. The optimum excitation and emission wavelengths were at lambda(ex)/lambda(em) = 270 nm/430 nm. Comparing with the widely used Dansyl-Cl, EASC has stronger photoluminescence characteristics(ultraviolet and fluorescence) : UV(EASC)/UV(Dansyl-Cl) = 3.2 (UV: ultraviolet absorption intensity); I(EASC/I(Dansyl-Cl) = 30.0: 1-105. 4:1 (I: relative fluorescence intensity); fluorescence quantum yield: Phi(f)(EASC)/Phi(f)(Dansyl-Cl) = 43.0:1. Fast and accurate quantitative analysis of aliphatic amines with fluorescence and corresponding post-column ion trap mass spectrometry(LC-APCI-MS) were obtained. EASC-amines exhibited stronger mass spectrometric sensitivity relative to that of dansyl-amine derivatives: MS(EASC)/MS(Dansyl-Cl) = 1. 6: 1-6. 2: 1 times(MS: relative intensity of ion current). The established method for the determination of aliphatic amines in environmental water samples exhibited good reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients >0.9995. The detection limits(at signal-to-noise of 3: 1) were at levels of 4.0-12.7 fmol.
关键词	High Performance Liquid Chromatography/ion-trap Mass Spectrometry Fluorescence Detection Pre-column Derivatization Aliphatic Amine 10-ethyl-9-oxo-9 10-dihydroacridine-2-sulfonyl Chloride
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; DANSYL CHLORIDE ; PRECOLUMN DERIVATIZATION ; FLUORESCENCE DETECTION ; DERIVATIVES ; IONIZATION
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000265665700007
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1886
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal Shandony, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao Huai-Xin,Sun Xue-Jun,Sun Zhi-Wei,et al. Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2009,30(4):675-681.
APA	Zhao Huai-Xin.,Sun Xue-Jun.,Sun Zhi-Wei.,Yan Tao.,Zhang Zai-Qing.,...&You Jin-Mao.(2009).Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,30(4),675-681.
MLA	Zhao Huai-Xin,et al."Determination of Free Aliphatic Amines in Environmental Water by EASC Derivatization and Identification with LC-APCI-MS".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 30.4(2009):675-681.