Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives

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	Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives
	Zhao Huai-Xin 1; Sun Xue-Jun 1; Sun Zhi-Wei 1; Hu Bao-Jun 1; Liu Qin-Ze 1; Zhao Xin-Quan 2; You Jin-Mao 1,2
	2009-02-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
ISSN	0253-3820
卷号	37 期号:2 页码:187-193
文章类型	Article
摘要	A novel labeling reagent 10-ethyl-acridine-2-sulfonyl chloride (EASC) was synthesized for the analysis of trace phenolic compound and a new highly sensitive method for the determination of estrogens such as estradiol and estriol using EASC as enhancing reagent with LC-APCI MS/MS (MRM) detection has been developed. Estradiol (extradiol) and estriol (extriol) could be labeled within 3 min at 60 degrees C in the presence of pH = 10. 5 NaHCO(3) buffer to give the corresponding sensitive MS ion current signal that could be detected by MS-APCI(MRM) in positive-ion mode. Compared with dansyl chloride, EASC exhibited excellent UV, fluourscence and MS properties: Ratio of molar absorptivity UV(EASC)/UV(Dansyl-Cl) = 6. 67, ratio of fluorescence quantum yield Phi(f)(EASC)/Phi(f) (Dansyl-Cl) = 43, ratio of ion intensity IC(EASC)/IC(Dansyl-Cl) = 4. 98 (extradiol); IC(EASC)/IC(Dansyl-Cl) = 4. 51 (extriol) (at the same derivative condition with extradiol and extriol At the same time, a detailed statement of the enhancing mechanism using EASC as MS labeling reagent was explained. The established method exhibited excellent repeatability and recovery. Linear responses in the range of 2.0 mu g/L - 100 mu g/L, for extradiol and extriol were observed with coefficients of >0.9995, and detection limits were 4. 3 and 14 ng/L (S/N = 3 : 1) for the labeled extradiol and extriol. The established method for the determination of trace free estradiol and estriol from feces sample of Tibetan antelope was satisfactory.; A novel labeling reagent 10-ethyl-acridine-2-sulfonyl chloride (EASC) was synthesized for the analysis of trace phenolic compound and a new highly sensitive method for the determination of estrogens such as estradiol and estriol using EASC as enhancing reagent with LC-APCI MS/MS (MRM) detection has been developed. Estradiol (extradiol) and estriol (extriol) could be labeled within 3 min at 60 degrees C in the presence of pH = 10. 5 NaHCO(3) buffer to give the corresponding sensitive MS ion current signal that could be detected by MS-APCI(MRM) in positive-ion mode. Compared with dansyl chloride, EASC exhibited excellent UV, fluourscence and MS properties: Ratio of molar absorptivity UV(EASC)/UV(Dansyl-Cl) = 6. 67, ratio of fluorescence quantum yield Phi(f)(EASC)/Phi(f) (Dansyl-Cl) = 43, ratio of ion intensity IC(EASC)/IC(Dansyl-Cl) = 4. 98 (extradiol); IC(EASC)/IC(Dansyl-Cl) = 4. 51 (extriol) (at the same derivative condition with extradiol and extriol At the same time, a detailed statement of the enhancing mechanism using EASC as MS labeling reagent was explained. The established method exhibited excellent repeatability and recovery. Linear responses in the range of 2.0 mu g/L - 100 mu g/L, for extradiol and extriol were observed with coefficients of >0.9995, and detection limits were 4. 3 and 14 ng/L (S/N = 3 : 1) for the labeled extradiol and extriol. The established method for the determination of trace free estradiol and estriol from feces sample of Tibetan antelope was satisfactory.
关键词	Liquid Chromatography-mass Spectrography 10-ethyl-acridine-2-sulfonyl Chloride Estradiol Estriol
WOS标题词	Science & Technology ; Physical Sciences
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; DANSYL CHLORIDE ; ELECTROSPRAY-IONIZATION ; DERIVATIZATION ; ESTRADIOL ; PLASMA ; SERUM
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000265169000005
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1896
专题	中国科学院西北高原生物研究所
作者单位	1.Qufu Normal Univ, Coll Chem Sci, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xinning 810001, Peoples R China
推荐引用方式 GB/T 7714	Zhao Huai-Xin,Sun Xue-Jun,Sun Zhi-Wei,et al. Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(2):187-193.
APA	Zhao Huai-Xin.,Sun Xue-Jun.,Sun Zhi-Wei.,Hu Bao-Jun.,Liu Qin-Ze.,...&You Jin-Mao.(2009).Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,37(2),187-193.
MLA	Zhao Huai-Xin,et al."Enhancing Sensitivity for Atmospheric Pressure Chemical Ionization Mass Spectrometry with a Novel Labeling Reagent: Application for the Analysis of Estrogens Derivatives".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 37.2(2009):187-193.