The Protein Level of Hypoxia-Inducible Factor-1 alpha Is Increased in the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes

NWIPB OpenIR

	The Protein Level of Hypoxia-Inducible Factor-1 alpha Is Increased in the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes
	Li, Hong-Ge 1,2; Ren, Yong-Ming 3; Guo, Song-Chang 1; Cheng, Long 3; Wang, De-Peng 1,2; Yang, Jie 1,2; Chang, Zhi-Jie 3; Zhao, Xin-Quan 1
	2009-02-01
发表期刊	JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY
ISSN	1932-5223
卷号	311A 期号:2 页码:134-141
文章类型	Article
摘要	The plateau pika (Ochotona curzoniae) is a high hypoxia-tolerant species living only at 3,000-5,000m above sea-level on the Qinghai-Tibetan plateau. Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor that regulates a variety of cellular and systemic adaptations to hypoxia. To investigate how the plateau pika adapts to a high-altitude hypoxic environment at the molecular level, we examined the expression pattern of the HIF-1 alpha protein in the pika by Western blot and immunohistochemical analysis. We found that HIF-1 alpha protein is expressed at a significantly high level in the pika, which is higher in most tissues (particularly in the lung, liver, spleen and kidney) of the plateau pika than that of mice living at sea-level. Importantly, we found that the protein levels of HIF-1 alpha in the lung, liver, spleen and kidney of the pika were increased with increased habitat altitudes. We observed that the plateau pika HIF-1 alpha localized to the nucleus of cells by an immunostaining analysis, and enhanced HRE-driven gene expression by luciferase reporter assays. Our study suggests that the HIF-1 alpha protein levels are related to the adaptation of the plateau pika to the high-altitude hypoxic environment. J. Exp. Zool. 311A:134-141, 2009. (c) 2008 Wiley-Liss, Inc.; The plateau pika (Ochotona curzoniae) is a high hypoxia-tolerant species living only at 3,000-5,000m above sea-level on the Qinghai-Tibetan plateau. Hypoxia-inducible factor-1 (HIF-1) is a key transcription factor that regulates a variety of cellular and systemic adaptations to hypoxia. To investigate how the plateau pika adapts to a high-altitude hypoxic environment at the molecular level, we examined the expression pattern of the HIF-1 alpha protein in the pika by Western blot and immunohistochemical analysis. We found that HIF-1 alpha protein is expressed at a significantly high level in the pika, which is higher in most tissues (particularly in the lung, liver, spleen and kidney) of the plateau pika than that of mice living at sea-level. Importantly, we found that the protein levels of HIF-1 alpha in the lung, liver, spleen and kidney of the pika were increased with increased habitat altitudes. We observed that the plateau pika HIF-1 alpha localized to the nucleus of cells by an immunostaining analysis, and enhanced HRE-driven gene expression by luciferase reporter assays. Our study suggests that the HIF-1 alpha protein levels are related to the adaptation of the plateau pika to the high-altitude hypoxic environment. J. Exp. Zool. 311A:134-141, 2009. (c) 2008 Wiley-Liss, Inc.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
关键词[WOS]	YAK BOS-GRUNNIENS ; ADULT-MOUSE BRAIN ; FACTOR 1-ALPHA ; MESSENGER-RNA ; CDNA CLONING ; HIF-ALPHA ; RAT-BRAIN ; EXPRESSION ; HYDROXYLATION ; HIF-1-ALPHA
收录类别	SCI
语种	英语
WOS研究方向	Zoology
WOS类目	Zoology
WOS记录号	WOS:000264315900008
引用统计	被引频次：28[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1898
专题	中国科学院西北高原生物研究所
作者单位	1.Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.Tsinghua Univ, Sch Med, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
推荐引用方式 GB/T 7714	Li, Hong-Ge,Ren, Yong-Ming,Guo, Song-Chang,et al. The Protein Level of Hypoxia-Inducible Factor-1 alpha Is Increased in the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes[J]. JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY,2009,311A(2):134-141.
APA	Li, Hong-Ge.,Ren, Yong-Ming.,Guo, Song-Chang.,Cheng, Long.,Wang, De-Peng.,...&Zhao, Xin-Quan.(2009).The Protein Level of Hypoxia-Inducible Factor-1 alpha Is Increased in the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes.JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY,311A(2),134-141.
MLA	Li, Hong-Ge,et al."The Protein Level of Hypoxia-Inducible Factor-1 alpha Is Increased in the Plateau Pika (Ochotona curzoniae) Inhabiting High Altitudes".JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY 311A.2(2009):134-141.