Extraction of pollen lipids by SFE-CO(2) and determination of free fatty acids by HPLC

NWIPB OpenIR

	Extraction of pollen lipids by SFE-CO(2) and determination of free fatty acids by HPLC
	Wang, XY; Wang, HL; Liu, YJ; You, JM; Suo, YR
	2009
发表期刊	EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
ISSN	1438-7697
卷号	111 期号:2 页码:155-163
摘要	Rape bee pollen lipids obtained by petrol ether extraction (PEE) or supercritical fluid (carbon dioxide) extraction (SFE) were compared with regard to their free fatty acid (FFA) components. Optimal SFE conditions were selected by carrying out the Taguchi method with an OA9 (3(3)) matrix design, and are as follows: extraction pressure at 35 MPa, temperature at 45 degrees C, and dynamic extraction time at 90 min. The lipid yield based on PEE was 7.42 wt-% and the extracts of the desired analytes based on SFE varied in the range of 3.23-5.58 wt-% under different conditions. With the optimized procedure, the lipid yield was 6.09 wt-%. The FFA in the lipids were separated with a pre-column derivation method and 1-[2-(p-toluensulfonate) ethyl]-2-phenylimidazole [4,5-f]9, 10-phenanthrene as labeling regent, followed by high-pressure liquid chromatography (HPLC) with fluorescence detection. analysis shows that the lipids contain abundant unsaturated fatty acids (UFA) in high to low concentrations as follows: linolenic acid (18:3), oleic acid (18:1), linoleic acid (18:2), nervonic acid (24:1), and lignoceric acid (20:4). The UFA contents in the SFE. extracts were higher than those after PEE. The results indicated that SFE under suitable conditions is more selective than conventional PEE with regard to lipid extraction and preservation of their quality.
关键词	Free Fatty Acids Hplc-fld Petrol Ether Extraction Rape Bee Pollen Lipid Supercritical Co(2) Extraction
收录类别	sci
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/1902
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, XY,Wang, HL,Liu, YJ,et al. Extraction of pollen lipids by SFE-CO(2) and determination of free fatty acids by HPLC[J]. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,2009,111(2):155-163.
APA	Wang, XY,Wang, HL,Liu, YJ,You, JM,&Suo, YR.(2009).Extraction of pollen lipids by SFE-CO(2) and determination of free fatty acids by HPLC.EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY,111(2),155-163.
MLA	Wang, XY,et al."Extraction of pollen lipids by SFE-CO(2) and determination of free fatty acids by HPLC".EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 111.2(2009):155-163.