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| Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent | |
| Zhiwei Sun; Jinmao You; Guoliang Li; Xian’en Zhao; Yourui Suo; Xiao Wang | |
| 2011-02-21 | |
| 发表期刊 | Journal of Chromatography B
 |
| ISSN | 1570-0232 |
| 卷号 | 879页码:1367-1374 |
| 摘要 | A high performance liquid chromatography method for the determination of 20 amino acids (AAs), using 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate (DBCEOC–Cl) as a novel fluorescent labeling reagent,has been developed and applied for the analysis of AAs in rat brain microdialysate. The simultaneous separation of 20 AA derivatives was achieved on a Hypersil BDS C18 column with gradient elution. And the identification of AA derivatives was carried out by on-line electrospray ionization mass spectrometry in positive ion mode. The AA derivatives were detected with excitation and emission at 300nmand 395 nm, respectively. Excellent linear responses were observed with coefficients of >0.9988. The detection limits ranged from 0.217 to 4.75 nmol/L, at a signal-to-noise ratio of 3. The intra-day and inter-day precision for each AA was <3.2% and <4.3%, respectively. The mean recoveries for all AAs studied were in the range of 92.5–105.6%. Good compositional data could be obtained from as little as 15_L of microdialysate. Facile DBCEOC–Cl derivatization coupled with high performance liquid chromatography and fluorescence detection allowed the development of a highly sensitive method for the quantitative analysis of trace levels of AAs from microdialysate. |
| 收录类别 | SCI |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://210.75.249.4/handle/363003/2004 |
| 专题 | 中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhiwei Sun,Jinmao You,Guoliang Li,et al. Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent[J]. Journal of Chromatography B,2011,879:1367-1374. |
| APA | Zhiwei Sun,Jinmao You,Guoliang Li,Xian’en Zhao,Yourui Suo,&Xiao Wang.(2011).Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent.Journal of Chromatography B,879,1367-1374. |
| MLA | Zhiwei Sun,et al."Determination of amino acids in rat brain microdialysate with 1,2,5,6-dibenzocarbazole-9-ethyl chloroformate as labeling reagent".Journal of Chromatography B 879(2011):1367-1374. |
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