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Development of a New HPLC Method with Pre-column Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits
GUO-LIANG LI; JIN-MAO YOU; CUI-HUA SONG; LIAN XIA; JIE ZHENG; YOU-RUI SUO
2011-03-09
Source Publicationjournal of agricultural and food chemistry
ISSN0021-8561
Volume59Issue:7Pages:2972-2979
AbstractTriterpenic acids are widespread in plants and have multiplicity of biological properties. Unfortunately the method for accurate analysis of these compounds remains poorly investigated. This study proposed a highly sensitive and selective pre-column derivatization method for accurate determination of five triterpenic acids (betulinic acid, betulonic acid, maslinic acid, ursolic acid and oleanolic acid) in fruits using acridone-9-ethyl-p-toluenesulfonate (AETS) as fluorescent labelling reagent by HPLC with fluorescence detection (FLD). Response surface methodology was employed to optimize the derivatization reaction, ensuring the sufficient labelling of the analyzed components. The rapid separation of five triterpenic acids could be achieved in as little as 16 min. This developed method offered the exciting detection limits of 1.68-2.04 ng/mL. When applied to several popular fruits in China, it revealed satisfactory applicability and reproducibility. This developed method also exhibits powerful potential for accurate detection of triterpenic acids from other foodstuffs and nature products.
Indexed BySCI
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/2008
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
GUO-LIANG LI,JIN-MAO YOU,CUI-HUA SONG,et al. Development of a New HPLC Method with Pre-column Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits[J]. journal of agricultural and food chemistry,2011,59(7):2972-2979.
APA GUO-LIANG LI,JIN-MAO YOU,CUI-HUA SONG,LIAN XIA,JIE ZHENG,&YOU-RUI SUO.(2011).Development of a New HPLC Method with Pre-column Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits.journal of agricultural and food chemistry,59(7),2972-2979.
MLA GUO-LIANG LI,et al."Development of a New HPLC Method with Pre-column Fluorescent Derivatization for Rapid, Selective and Sensitive Detection of Triterpenic Acids in Fruits".journal of agricultural and food chemistry 59.7(2011):2972-2979.
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