Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells

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	Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells
	Shi, Ping ; Huang, Zhiwei ; Chen, Guichen
	2008
发表期刊	AMERICAN JOURNAL OF CHINESE MEDICINE ; Shi Ping,Huang Zhiwei,Chen Guichen. Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.AMERICAN JOURNAL OF CHINESE MEDICINE,2008,36(4):805-813
摘要	Rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. In this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma BEL-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. Using MTT assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of BEL-7402 cell proliferation in a concentration- and time-dependent manner. The increase of apoptosis rate was observed after incubation of BEL-7402 cells with rhein at 50-200 mu M for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. Moreover, rhein-induced cell cycle S-phase arrest. Additionally, after rhein treatment, expression levels of c-Myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time PCR assay. The results demonstrate for the first time that cell cycle S-phase arrest is one of the mechanisms of rhein in inhibition of BEL-7402 cells. Rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-Myc and apoptosis through the caspase-dependent pathway. It is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future.; Rhein, an anthraquinone derivative of rhubarb, inhibits the proliferation of various human cancer cells. In this paper, we focused on studying the effects of rhein on human hepatocelluar carcinoma BEL-7402 cells and further understanding the underlying molecular mechanism in an effort to make the potential development of rhein in the treatment of cancers. Using MTT assay and flow cytometry, we demonstrate a critical role of rhein in the suppression of BEL-7402 cell proliferation in a concentration- and time-dependent manner. The increase of apoptosis rate was observed after incubation of BEL-7402 cells with rhein at 50-200 mu M for 48 hours, and the cells exhibit typical apoptotic features including cellular morphological change and chromatin condensation. Moreover, rhein-induced cell cycle S-phase arrest. Additionally, after rhein treatment, expression levels of c-Myc gene were decreased, while those of caspase-3 gene were increased in a dose-dependent manner by using real-time PCR assay. The results demonstrate for the first time that cell cycle S-phase arrest is one of the mechanisms of rhein in inhibition of BEL-7402 cells. Rhein plays its role by inducing cell cycle arrest via downregulation of oncogene c-Myc and apoptosis through the caspase-dependent pathway. It is expected that rhein will be effective and useful as a new agent in hepatocelluar carcinoma treatment in the future.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20129
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Shi, Ping,Huang, Zhiwei,Chen, Guichen. Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells[J]. AMERICAN JOURNAL OF CHINESE MEDICINE, Shi Ping,Huang Zhiwei,Chen Guichen. Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.AMERICAN JOURNAL OF CHINESE MEDICINE,2008,36(4):805-813,2008.
APA	Shi, Ping,Huang, Zhiwei,&Chen, Guichen.(2008).Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells.AMERICAN JOURNAL OF CHINESE MEDICINE.
MLA	Shi, Ping,et al."Rhein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma BEL-7402 cells".AMERICAN JOURNAL OF CHINESE MEDICINE (2008).