LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification

NWIPB OpenIR

	LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification
	Kang, Li ; You, Jinmao ; Sun, Zhiwei ; Wang, Chengyan ; Ji, Zhongyin ; Gao, Yongping ; Suo, Yourui ; Li, Yulin
	2011
发表期刊	CHROMATOGRAPHIA ; Kang, L; You, JM; Sun, ZW; Wang, CY; Ji, ZY; Gao, YP; Suo, YR; Li, YL.LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification,CHROMATOGRAPHIA,2011,73(1-2):43-50
摘要	A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M + H](+) in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol L(-1) with coefficients of > 0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.; A pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M + H](+) in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02-10 mu mol L-1 with coefficients of > 0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were < 0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7-103.6% for chicken sausage and 95.8-104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20478
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Kang, Li,You, Jinmao,Sun, Zhiwei,et al. LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification[J]. CHROMATOGRAPHIA, Kang, L; You, JM; Sun, ZW; Wang, CY; Ji, ZY; Gao, YP; Suo, YR; Li, YL.LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification,CHROMATOGRAPHIA,2011,73(1-2):43-50,2011.
APA	Kang, Li.,You, Jinmao.,Sun, Zhiwei.,Wang, Chengyan.,Ji, Zhongyin.,...&Li, Yulin.(2011).LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification.CHROMATOGRAPHIA.
MLA	Kang, Li,et al."LC Determination of Trace Biogenic Amines in Foods Samples with Fluorescence Detection and MS Identification".CHROMATOGRAPHIA (2011).