HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau

NWIPB OpenIR

	HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau
	Yue, Pengpeng ; Sun, Jing ; Zhang, Changxian ; Ye, Runrong ; Lu, Xuefeng ; Zhou, Yubi ; Yang, Shibing ; Peng, Min
	2010-06-04
发表期刊	JOURNAL OF MEDICINAL PLANTS RESEARCH ; Yue, PP; Sun, J; Zhang, CX; Ye, RR; Lu, XF; Zhou, YB; Yang, SB; Peng, M.HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau,JOURNAL OF MEDICINAL PLANTS RESEARCH,2010,4(11):1053-1058
摘要	This study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).; This study describes a simple and sensitive method for the simultaneous determination of major flavonoids active constituents in a traditional Tibetan medicine Meconopsis quintuplinervia collecting from 29 different localities of Qinghai-Tibet Plateau by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). The optimal conditions of separation and detection were achieved on a Zorbax Eclipse XDB-C(18) column (4.6 x 250 mm, 5 mu m) with a gradient of methanol and 0.05% aqueous acetic acid (v/v), at a flow rate of 1.0 ml min(-1), detected at 370 nm. The complete separation was obtained within 40 min for the four analytes. The recovery of the method was >100%, and all the flavonoids showed good linearity (r >= 0.9990) in a relatively wide concentration range. The limits of detection (LOD) are (injection volume 10 mu l, at a signal-to-noise ratio of 3, S/N = 3:1) between 0.09 and 0.36 mu g ml(-1). Furthermore, the main characteristics of flavonoids in M. quintuplinervia were also analyzed by principle component analysis (PCA).
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20516
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yue, Pengpeng,Sun, Jing,Zhang, Changxian,et al. HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau[J]. JOURNAL OF MEDICINAL PLANTS RESEARCH, Yue, PP; Sun, J; Zhang, CX; Ye, RR; Lu, XF; Zhou, YB; Yang, SB; Peng, M.HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau,JOURNAL OF MEDICINAL PLANTS RESEARCH,2010,4(11):1053-1058,2010.
APA	Yue, Pengpeng.,Sun, Jing.,Zhang, Changxian.,Ye, Runrong.,Lu, Xuefeng.,...&Peng, Min.(2010).HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau.JOURNAL OF MEDICINAL PLANTS RESEARCH.
MLA	Yue, Pengpeng,et al."HPLC-DAD separation and determination of major active constituents in an important Tibetan medicine Meconopsis quintuplinervia from different regions of Qinghai-Tibet Plateau".JOURNAL OF MEDICINAL PLANTS RESEARCH (2010).