Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent

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	Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent
	Yan Tao ; Sun Xue-Jun ; Zhao Huai-Xin ; Sun Zhi-Wei ; Liu Qin-Ze ; Suo You-Rui ; You Jin-Mao
	2009-06-01
发表期刊	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ; Yan, T; Sun, XJ; Zhao, HX; Sun, ZW; Liu, QZ; Suo, YR; You, JM.Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(6):788-794
摘要	On a reversed-phase Eclipse XDB C8 ( 150 mm x 4.6 mm, 5 mu m) column, 12 aliphatic amine derivatives were derivatized using 2-[2-(7H-dibenzo[a, g] carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase. Detection was carried out by the fluorescence and online post-column atmospheric pressure chemical ionization(APCI) in positive-ion mode. The maximum excitation and emission wavelengths were at 300 nm and 400 nm, respectively. Identification of aliphatic amine derivatives was carried out by the online post-column Ion trap/mass spectrometry with positive-ion detection mode of APCI-MS. A rapid, accurate method for the determination of free aliphatic amine extracted from soil was proposed. The established method exhibited excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0.9991. The detection limits( at signal-to-noise of 3:1) were 10.1-0.3 fmol.; On a reversed-phase Eclipse XDB C8 ( 150 mm x 4.6 mm, 5 mu m) column, 12 aliphatic amine derivatives were derivatized using 2-[2-(7H-dibenzo[a, g] carbazol-7-yl)-ethoxy] ethyl chloroformate (DBCEC-Cl) as a new pre-column derivatization reagent. The complete baseline resolution was obtained in conjunction with a gradient elution with acetonitrile/H(2)O as mobile phase. Detection was carried out by the fluorescence and online post-column atmospheric pressure chemical ionization(APCI) in positive-ion mode. The maximum excitation and emission wavelengths were at 300 nm and 400 nm, respectively. Identification of aliphatic amine derivatives was carried out by the online post-column Ion trap/mass spectrometry with positive-ion detection mode of APCI-MS. A rapid, accurate method for the determination of free aliphatic amine extracted from soil was proposed. The established method exhibited excellent reproducibility and recovery. Excellent linear responses were observed with the correlation coefficients > 0.9991. The detection limits( at signal-to-noise of 3:1) were 10.1-0.3 fmol.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/20608
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Yan Tao,Sun Xue-Jun,Zhao Huai-Xin,et al. Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, Yan, T; Sun, XJ; Zhao, HX; Sun, ZW; Liu, QZ; Suo, YR; You, JM.Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent,CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2009,37(6):788-794,2009.
APA	Yan Tao.,Sun Xue-Jun.,Zhao Huai-Xin.,Sun Zhi-Wei.,Liu Qin-Ze.,...&You Jin-Mao.(2009).Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA	Yan Tao,et al."Mass Spectrometric Identification of Aliphatic Amines Using 2-[2-(7H-dibenzo[a,g]-carbazol-7-yl)-ethoxy] ethyl Chloroformate as Novel Fluorescence Labeling Reagent".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2009).