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LC Determination of Trace Biogenic Amines in Foods Samples
Li Kang; Jinmao You; Zhiwei Sun; Chengyan Wang; Zhongyin Ji; Yongping Gao; Yourui Suo; Yulin Li
2011-01-08
Source PublicationChromatographia
ISSN0009-5893
Volume73Pages:43-50
AbstractA pre-column derivatization method for the simple, sensitive determination of biogenic amines using 10-ethyl-acridine-3-sulfonyl chloride (EASC) as labeling reagent with fluorescence detection and mass spectrometry (MS) identification has been developed. After pre-column derivatization, the labeled biogenic amines were separated on a Hypersil BDS-C18 column by gradient elution. The derivatives showed an intense protonated molecular ion corresponding m/z [M ? H]? in positive-ion mode. The collision-induced dissociation of protonated molecular ion formed specific fragment ions at m/z 196.5, m/z 222.7, m/z 224.4 and m/z 272.5, m/z 286.2. Satisfactory linear responses were observed at the concentration range of 0.02–10 lmol L-1 with coefficients of[0.9993. Detection limits obtained by the analysis of a derivatized standard containing 0.2 pmol of each biogenic amine, were from 20.22 to 109.2 fmol (at a signal-to-noise ratio of 3). The relative standard deviations of retention times and peak areas for each biogenic amine were \0.96 and 3.22%, respectively. Recoveries except for PUT were in the range of 96.7–103.6% for chicken sausage and 95.8–104.6% for pork sausage The established method for the determination of biogenic amines except for PUT from real samples was satisfactory.
Indexed BySCI
Document Type期刊论文
Identifierhttp://210.75.249.4/handle/363003/2138
Collection中国科学院西北高原生物研究所
Recommended Citation
GB/T 7714
Li Kang,Jinmao You,Zhiwei Sun,et al. LC Determination of Trace Biogenic Amines in Foods Samples[J]. Chromatographia,2011,73:43-50.
APA Li Kang.,Jinmao You.,Zhiwei Sun.,Chengyan Wang.,Zhongyin Ji.,...&Yulin Li.(2011).LC Determination of Trace Biogenic Amines in Foods Samples.Chromatographia,73,43-50.
MLA Li Kang,et al."LC Determination of Trace Biogenic Amines in Foods Samples".Chromatographia 73(2011):43-50.
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