Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants

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	Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants
	Jiang, Yuanzhong ; Ye, Shenglong ; Wang, Lijun ; Duan, Yanjiao ; Lu, Wanxiang ; Liu, Hong ; Fan, Di ; Zhang, Faqi ; Luo, Keming
	2014-02-01
发表期刊	PLANT CELL TISSUE AND ORGAN CULTURE ; Jiang, YZ; Ye, SL; Wang, LJ; Duan, YJ; Lu, WX; Liu, H; Fan, D; Zhang, FQ; Luo, KM.Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants,PLANT CELL TISSUE AND ORGAN CULTURE,2014,116(2):163
摘要	Virus-induced gene silencing (VIGS) is an effective tool for studying the functions of plant genes, but only a few VIGS vectors available for woody plants were reported so far. Here we present an effective heterologous VIGS system in woody plants based on tobacco rattle virus (TRV) vectors. We first tested whether the TRV-vector can be directly applied to infect woody plant species, such as Vernicia fordii, Populus tomentosa Carr. and Camellia oleifera. The results revealed that TRV-mediated VIGS could be effectively elicited in V. fordii, weakly in P. tomentosa Carr., but not in C. oleifera. TRV-based VIGS vectors with heterologous phytoene desaturase (PDS) sequences from various woody plant species silenced successfully the endogenous PDS gene in Nicotina benthamiana and V. fordii. The photobleached leaf phenotype of silenced plants significantly correlated with the down-regulation of endogenous PDS as compared with controls. To further confirm the reliability of VIGS in V. fordii, we also isolated the cloroplastos alterados 1 gene from P. tomentosa Carr., and the silencing pheotypes of albino leaves were observed in V. fordii 2 weeks after inoculation using a heterologous TRV-based VIGS system. Taken together, we have successfully developed an Agrobacterium-mediated VIGS assay in V. fordii and demonstrated that V. fordii as a heterologous VIGS system provides a valuable tool for functional genomic analysis in woody plant species.; Virus-induced gene silencing (VIGS) is an effective tool for studying the functions of plant genes, but only a few VIGS vectors available for woody plants were reported so far. Here we present an effective heterologous VIGS system in woody plants based on tobacco rattle virus (TRV) vectors. We first tested whether the TRV-vector can be directly applied to infect woody plant species, such as Vernicia fordii, Populus tomentosa Carr. and Camellia oleifera. The results revealed that TRV-mediated VIGS could be effectively elicited in V. fordii, weakly in P. tomentosa Carr., but not in C. oleifera. TRV-based VIGS vectors with heterologous phytoene desaturase (PDS) sequences from various woody plant species silenced successfully the endogenous PDS gene in Nicotina benthamiana and V. fordii. The photobleached leaf phenotype of silenced plants significantly correlated with the down-regulation of endogenous PDS as compared with controls. To further confirm the reliability of VIGS in V. fordii, we also isolated the cloroplastos alterados 1 gene from P. tomentosa Carr., and the silencing pheotypes of albino leaves were observed in V. fordii 2 weeks after inoculation using a heterologous TRV-based VIGS system. Taken together, we have successfully developed an Agrobacterium-mediated VIGS assay in V. fordii and demonstrated that V. fordii as a heterologous VIGS system provides a valuable tool for functional genomic analysis in woody plant species.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/22123
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Jiang, Yuanzhong,Ye, Shenglong,Wang, Lijun,et al. Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants[J]. PLANT CELL TISSUE AND ORGAN CULTURE, Jiang, YZ; Ye, SL; Wang, LJ; Duan, YJ; Lu, WX; Liu, H; Fan, D; Zhang, FQ; Luo, KM.Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants,PLANT CELL TISSUE AND ORGAN CULTURE,2014,116(2):163,2014.
APA	Jiang, Yuanzhong.,Ye, Shenglong.,Wang, Lijun.,Duan, Yanjiao.,Lu, Wanxiang.,...&Luo, Keming.(2014).Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants.PLANT CELL TISSUE AND ORGAN CULTURE.
MLA	Jiang, Yuanzhong,et al."Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants".PLANT CELL TISSUE AND ORGAN CULTURE (2014).