Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants

NWIPB OpenIR

	Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants
	Ye, Shenglong ; Jiang, Yuanzhong ; Duan, Yanjiao ; Karim, Abdul ; Fan, Di ; Yang, Li ; Zhao, Xin ; Yin, Jia ; Luo, Keming ; Luo, KM (reprint author), Southwest Univ, Chongqing Key Lab Transgen Plant & Safety Control, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, 2 Tiansheng Rd, Chongqing 400715, Peoples R China.
	2014-10-01
发表期刊	TREE PHYSIOLOGY
摘要	WRKY proteins are involved in various physiological processes in plants, especially in coping with diverse biotic and abiotic stresses. However, limited information is available on the roles of specific WRKY transcription factors in poplar defense. In this study, we reported the characterization of PtoWRKY60, a Group IIa WRKY member, from Populus tomentosa Carr. The gene expression profile of PtoWRKY60 in various tissues showed that it significantly accumulated in old leaves. Phylogenetic analyses revealed that PtoWRKY60 had a close relationship with AtWRKY18, AtWRKY40 and AtWRKY60. PtoWRKY60 was induced mainly by salicylic acid (SA) and slightly by Dothiorella gregaria Sacc., jasmonic acid, wounding treatment, low temperature and salinity stresses. Overexpression of PtoWRKY60 in poplar resulted in increased resistance to D. gregaria. The defense-associated genes, such as PR5.1, PR5.2, PR5.4, PR5.5 and CPR5, were markedly up-regulated in transgenic plants overexpressing PtoWRKY60. These results indicate that PtoWRKY60 might be partly involved in the signal transduction pathway initiated by SA in Populus.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/22848
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Ye, Shenglong,Jiang, Yuanzhong,Duan, Yanjiao,et al. Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants[J]. TREE PHYSIOLOGY,2014.
APA	Ye, Shenglong.,Jiang, Yuanzhong.,Duan, Yanjiao.,Karim, Abdul.,Fan, Di.,...&Luo, KM .(2014).Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants.TREE PHYSIOLOGY.
MLA	Ye, Shenglong,et al."Constitutive expression of the poplar WRKY transcription factor PtoWRKY60 enhances resistance to Dothiorella gregaria Sacc. in transgenic plants".TREE PHYSIOLOGY (2014).