Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection

NWIPB OpenIR

	Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection
	Wang, Yuwei ; Suo, Yourui ; Sun, Yanan ; You, Jinmao
	2016-11-01
发表期刊	CHROMATOGRAPHIA
摘要	In this work, we have developed an efficient method for the rapid extraction and separation of triterpene acids from 37 different varieties of raspberry via ultrasound-assisted dispersive liquid-liquid microextraction (UA-DLLME). The triterpene acids were then determined by high-performance liquid chromatography (HPLC) with fluorescence detection using benzimidazo-[2,1-b]quinazolin-12(6H)-one-5-ethyl-p-toluenesulfonate (BQETS) as the labeling agent. Five triterpene acids, including asiatic acid (AA), maslinic acid (MA), corosolic acid (CA), oleanolic acid (OA) and betulinic acid (BA), were extracted by UA-DLLME using chloroform and acetone as the extracting and dispersing solvents, respectively. After extraction and nitrogen flushing, the extracts were simultaneously characterized by HPLC based on pre-column derivatization using BQETS, a new labeling agent synthesized in our laboratory. Several key parameters affecting the extraction efficiency and derivatization yields were investigated and optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD). The method was further validated for linearity (correlation coefficient R (2) > 0.9979), precision (RSD = 0.23-2.45 %), and recovery (RSD = 90-106.5 %). The limits of detection (LODs) and the limits of quantification (LOQs) were determined to be within the range of 1.83-7.69 A mu g/L and 6.06-25.47 A mu g/L, respectively. This is the first report of the use of BQETS as a pre-column derivatization agent for the determination of triterpene acids in real samples. The proposed method has been applied to the determination of five triterpene acids in 37 different raspberry varieties with significantly increased sensitivity compared to other methods. The results obtained indicate that the contents of triterpene acids vary significantly across different raspberry varieties.
文献类型	期刊论文
条目标识符	http://210.75.249.4/handle/363003/22887
专题	中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Wang, Yuwei,Suo, Yourui,Sun, Yanan,et al. Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection[J]. CHROMATOGRAPHIA,2016.
APA	Wang, Yuwei,Suo, Yourui,Sun, Yanan,&You, Jinmao.(2016).Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection.CHROMATOGRAPHIA.
MLA	Wang, Yuwei,et al."Determination of Triterpene Acids from 37 Different Varieties of Raspberry using Pre-column Derivatization and HPLC Fluorescence Detection".CHROMATOGRAPHIA (2016).