NWIPB OpenIR
Determination of neurotransmitters in rat telencephalon using pre-column fluorescence derivatization with high performance liquid chromatography/mass spectrometry
Zhao Xian-En ; Suo You-Rui
2008
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
摘要A sensitive method has been developed for the simultaneous determination of 5 neurotransmitters glutamic acid, gamma-amino-butyric acid, dopamine, 5-hydroxytryptamine, 5-hydroxyindole acetic acid) with I, 2-benzo-3, 4-dihydrocarbazole-9-ethyl chloroformate (BCEOC) as derivatization reagent on a reversed-phase Hypersil BDS C-18 column (100 mm x 4.6 mm, 5 mu m) with a gradient elution followed by high performance liquid chromatography (HPLC) via fluorescence detection at 390 nm (excitation wavelength 333 nm) and online mass spectrometric identification. The linear range of 5 neurotransmitters was 24 fmol - 200 pmol, all the correlation coefficients were > 0.9997, the detection limit was 4.0 - 12.6 fmol (at a signal to noise ratio of 3: 1). The MS identification of 5 neurotransmitter derivatives was carried out by post-column online mass spectrometry with electrospray ion source, the MS/MS fragment ions of them were listed, and the MS/MS cleavage mode of representative 5-hydroxytryptamine derivative was analyzed. Under all the above optimum experimental conditions, the contents of 5 neurotransmitters in telencephalon of 64 rat (classified as 2 groups, A Group: quiet contrast group, B Group: training group) at 4 states (a: quiet state, b: 1 h after exercise, c: at exercising exhaust, d: 12 h after exercising exhaust) were determined. It is indicated that a long period of time medium intensity training can effectively improve mice exercise capacity and obviously promote to restore central fatigue after resistance exercise. The established method exhibits high sensitivity and excellent reproducibility, and provides a new technology for the simultaneous determination of monoamine and amino acid neurotransmitters in rat telencephalon.
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/23027
专题中国科学院西北高原生物研究所
推荐引用方式
GB/T 7714
Zhao Xian-En,Suo You-Rui. Determination of neurotransmitters in rat telencephalon using pre-column fluorescence derivatization with high performance liquid chromatography/mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008.
APA Zhao Xian-En,&Suo You-Rui.(2008).Determination of neurotransmitters in rat telencephalon using pre-column fluorescence derivatization with high performance liquid chromatography/mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY.
MLA Zhao Xian-En,et al."Determination of neurotransmitters in rat telencephalon using pre-column fluorescence derivatization with high performance liquid chromatography/mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (2008).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhao Xian-En]的文章
[Suo You-Rui]的文章
百度学术
百度学术中相似的文章
[Zhao Xian-En]的文章
[Suo You-Rui]的文章
必应学术
必应学术中相似的文章
[Zhao Xian-En]的文章
[Suo You-Rui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。