NWIPB OpenIR
Selectively screen the antibacterial peptide from the hydrolysates of highland barley
Pei, Jinjin ; Feng, Zhenzhen ; Ren, Ting ; Jin, Wengang ; Li, Xinsheng ; Chen, Dejing ; Tao, Yanduo ; Dang, Jun
2018
发表期刊ENGINEERING IN LIFE SCIENCES
摘要Highland barley is one of the most important industrial crops in Tibetan plateau. Previous research indicated that highland barley has many medical functions. In this work, the antibacterial abilities of highland barley were investigated. The protein solutions hydrolyzed by trypsin for 4 h exhibited the highest antibacterial activity. An antibacterial peptide, barleycin, was screened and purified by magnetic liposome extraction combining with the protein profiles of reversed-phase high-performance liquid chromatography (RP-HPLC). Structure, characterization, and safety evaluation of barleycin were further investigated. Amino acids sequence was determined as Lys-Ile-Ile-Ile-Pro-Pro-Leu-Phe-His by N-sequencing. Circular dichroism spectra indicated the a-helix conformation of barleycin. The activity spectrum included Bacillus subtilis, Staphylcoccus aureus, Listeria innocua and Escherichia coli and the MICs were from 4 to 16g/mL. Safety evaluations with cytotoxicity and hemolytic suggested this antibacterial peptide could be considered as safe at MICs. Finally, mode of action of barleycin on sensitive cells was primarily studied. The results suggested the damage of cell membrane.
出版者WILEY
文献类型期刊论文
条目标识符http://210.75.249.4/handle/363003/23745
专题中国科学院西北高原生物研究所
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GB/T 7714
Pei, Jinjin,Feng, Zhenzhen,Ren, Ting,et al. Selectively screen the antibacterial peptide from the hydrolysates of highland barley[J]. ENGINEERING IN LIFE SCIENCES,2018.
APA Pei, Jinjin.,Feng, Zhenzhen.,Ren, Ting.,Jin, Wengang.,Li, Xinsheng.,...&Dang, Jun.(2018).Selectively screen the antibacterial peptide from the hydrolysates of highland barley.ENGINEERING IN LIFE SCIENCES.
MLA Pei, Jinjin,et al."Selectively screen the antibacterial peptide from the hydrolysates of highland barley".ENGINEERING IN LIFE SCIENCES (2018).
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